热休克蛋白70与肝脏缺血预处理延迟保护效应  

Function of HSP70 in late preconditioning in ischemia reperfusion injury in rat liver

作　　者：王占明[1] 鲁建国[1] 赖大年[1] 包国强[1] 马庆久[1] 吴金生[1] 

机构地区：[1]第四军医大学唐都医院普通外科,陕西西安710038

出　　处：《第四军医大学学报》2001年第13期1200-1203,共4页Journal of the Fourth Military Medical University

摘　　要：目的　研究缺血预处理对大鼠肝脏缺血再灌注损伤后延迟保护效应的机制 .方法　建立大鼠肝脏缺血再灌注模型并进行缺血预处理 ,实验分组如下 :1假手术组 ( Sham - op-eration,S组 ) ;2缺血再灌注组 ( ischem ic reperfusion,I/ R组 ) ;3缺血预处理组 ( ischem ic preconditioning,PC组 ) ;4预处理加左旋硝基精氨酸组 ( preconditioning+ L - NNA,PC+ L组 ) ,各组再灌注后检测血清谷丙转氨酶 ( alanine aminotrans-ferase,AL T)及丙二醛 ( malondialdehyde,MDA )含量、热休克蛋白 70 ( HSP70 )免疫组化染色 ,肝组织石蜡切片 HE染色及电镜观察 .结果　 PC组 AL T及 MDA含量明显低于 I/ R组 ( P<0 .0 1) ,肝组织损伤程度明显减轻 ;PC+ L组在 0～ 3 h阶段 AL T和 MDA含量与 I/ R组无显著区别 ( P>0 .0 5 ) ,而在 6～ 4 8h阶段则显著低于 I/ R组 ( P<0 .0 1)但仍高于 PC组( P<0 .0 5 ) .HSP70免疫组化染色 :PC组、PC+ L 组 3～ 4 8h染色阳性率逐渐增多并显著高于 I/ R组 ( P<0 .0 1) .结论　NO和 HSP70均对大鼠肝脏缺血再灌注损伤具有保护作用 ,HSP70可能是导致延迟保护效应的重要因素 .AIM To assess the protection of HSP70's and time course in the preconditioning (PC) in ischemia/reperfu sion (I/R) injury in rat liver. METHODS 168 SD rats were randomly divided into four groups: ① S group: n =42, sham operated control; ② I/R group: n =42, subjected to ischemia for 70 min; ③ PC group: n =42, ischemia for 5 min and reperfusion for 5 min for twice, then subjected to ischemia; ④ PC+L group: n =42, the rats were administered L NNA (10 mg·kg -1 ) before preconditioning, and then were subjected to ischemia for 70 min. Serum level of alanine aminotransferase (ALT) and malondialdehyde (MDA) was detected during reperfution. Expression of HSP70 in hepatic cell was analysed by histochemical technique. RESULTS Serum level of ALT and MDA of both I/R and PC+L group increased, compared with PC group ( P <0.01) after reperfusion. There was not significant difference between I/R group and PC+L group during 0~3 h ( P >0.05) but there was during 6~48 h ( P <0.01). The serum level of ALT and MDA was higher in PC+L vs PC group ( P <0.05) but lower than I/R group during 6~48 h ( P <0.01). Expression level of HSP70 in PC and PC+L group became gradually higher than I/R group during 6~48 h ( P <0.01). CONCLUSION NO and HSP70 might be involved in the protection against liver ischemia/reperfusion injury and HSP70 might be associated with the late preconditioning.

关 键 词：缺血预处理 热休克蛋白70 肝缺血 再灌注损伤 免疫组织化学 

分 类 号：R657.3[医药卫生—外科学]