慢性庆大霉素损伤后豚鼠前庭感觉上皮蛋白激酶C表达和活性  

PKC expression and activity after chronic gentamicin toxicity in vestibular sensory epithelium of guinea pigs

作　　者：马晓莉[1] 姜鸿彦[1] 黄维国[1] 邱建华[1] 王锦玲[1] 刘顺利[1] 

机构地区：[1]第四军医大学西京医院耳鼻咽喉科,陕西西安710033

出　　处：《第四军医大学学报》2001年第13期1207-1210,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 ( 39870 788)

摘　　要：目的　研究庆大霉素慢性损伤后豚鼠前庭终器蛋白激酶 C( PKC)活性的变化及其β ,β ,γ亚型的表达 ,探讨PKC与前庭损伤修复的关系 .方法　采用免疫细胞化学法观察椭圆囊斑、球囊斑和壶腹嵴 PKCβ , ,γ亚型的表达 ;应用γ- 32 P示踪检测前庭终器 PKC活性的变化 .结果　 PKCβ , ,γ亚型在前庭感觉上皮细胞均呈阳性表达 ,上皮下结缔组织呈阴性表达 .β ,γ亚型较 β1 亚型表达稍弱 ,不同亚型抗原分布不完全相同 .与对照组相比 ,PKCβ ,γ在停止注射庆大霉素后 1d其表达明显减弱 ,随后其表达逐渐增加 ,至 1wk其表达已高于对照组 ,3 wk仍未恢复 .PKCβ 表达各组变化不明显 .豚鼠前庭终器 PKC活性检测结果与 PKCβ ,γ表达变化规律基本相似 .结论　庆大霉素慢性中毒后豚鼠前庭感觉上皮 PKCβ ,γ亚型表达及 PKC活性均发生变化 ,且不同亚型抗原分布不完全相同 .提示 PKC在前庭感觉上皮损伤修复过程中发挥重要作用 ,不同亚型可能具有不同的作用 .AIM To investigate the PKC expression and activity after chronic gentamicin toxicity in vestibular terminalorgans of guinea pigs and to study the relations between PKC and injury repair process of vestibule. METHODS By using immunohistochemical method, the expressions of PKCβ Ⅰ and PKCγ were examined in ginea pig macula utriculus, macula sacculus and crista ampullaris. The γ 32 P was used as a tracer to measure the activity of PKC in vestibular terminal organs. RESULTS The positive expression of PKC isoforms β Ⅰ,Ⅱ and γ was detected in vestibular sensory epithelium. There was negative staining in connective tissue lying under epithelium. The stain of PKC isoforms β Ⅱ and γ was weaker than that of PKCβ Ⅰ. The antigen distributions of different PKC isoforms were different. In comparison with control group, the expression of PKCβ Ⅰ and γ decreased at 1 d after injection of gentamicin and then it increased gradually. The expression was obviously higher than control group at 1 wk after injection and it still did not recover at 3 wk after injection. There were no obvious differences in expressions of PKCβ Ⅱ among different groups. The change trend of PKC activity was similar to the expression change of PKCβ Ⅰ and γ as a whole. CONCLUSION There were some changes of PKC expression and activity in vestibular terminal organs of guinea pigs after chronic gentamicin toxicity and the antigen distributions of different PKC isoforms were different. PKC may play a significant role in injury repair process of vestibule sensory epithelium and different PKC isoforms may have different physiological functions respectively.

关 键 词：庆大霉素 前庭 感觉上皮 蛋白激酶C 豚鼠 

分 类 号：R764.5[医药卫生—耳鼻咽喉科] R978.12[医药卫生—临床医学]