黎氏哮喘Ⅰ号方对哮喘小儿环核苷酸及哮喘豚鼠肺超微结构的影响  

Effects of Lishi Xiaochuan I on Cyclic Nucleotide in Asthmatic Children and Ultrastructure of Pulmonary Capillary Wall in Asthmatic Guinea Pigs

作　　者：黎世明[1] 谢昭亮[1] 黄永强[1] 吉训超[1] 李圳英[1] 陈芝喜[2] 刘 松 刘 华[4] 

机构地区：[1]广州中医药大学第一附属医院儿科,广州510405 [2]广州中医药大学核医学室,广州510405 [3]广州市东山区中医院,广州510080 [4]广州中医药大学,98级硕士生广州510405

出　　处：《广州中医药大学学报》2001年第3期232-236,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局资助项目(编号:97196)

摘　　要：[目的]探讨小儿哮喘与血浆环核苷酸的关系及黎氏哮喘Ⅰ号方(由麻黄、桂枝、五味子、鹅管石、当归、毛冬青、五指毛桃根等组成)治疗哮喘的作用机理。[方法]将70例哮喘患儿随机分为黎氏哮喘Ⅰ号方治疗组(A)47例和氨茶碱对照组(B)23例,并设10例健康儿童对照组(C),应用放射免疫法观察各组治疗前后血浆cAMP(环磷酸腺苷)、cGMP(环磷酸鸟苷)水平,并在哮喘豚鼠模型上进行验证。同时利用透射式电子显微镜观察黎氏哮喘Ⅰ号方对哮喘豚鼠肺超微结构变化的影响。[结果]治疗后A、B两组哮喘儿童cAMP、cGMP水平均有改善,cAMP/cGMP比值上升(与治疗前相比,均P<0.01)。动物实验表明,治疗后中药高剂量组cAMP/cGMP比值改善优于氨茶碱组(P<0.01)。黎氏哮喘Ⅰ号方连续治疗7 d后,哮喘豚鼠肺毛细血管壁增厚程度减轻,无胶原纤维增生。[结论]黎氏哮喘Ⅰ号方能提高哮喘患儿及哮喘豚鼠cAMP/cGMP比值,减轻哮喘豚鼠肺组织的炎症程度。ive] To explore the mechanism of Lishi Xiaochuan I for asthma in children. [Meth ods] Seventy children suffering asthma were randomly allocated to Lishi Xiaochuan I group (Group A, n = 47) and aminophylline group (Group B), and ten healthy children served as the control (Group C). Radioimmunoassay was used to examine the levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) before and after treatment. Meanwhile, effect of Lishi Xiaochuan I on pulmonary ultrastructure of asthmatic guinea pigs was observed by transmission electron microscope. [Results] cAMP level was increased and cGMP level decreased, and the ratio of cAMP/cGMP increased after treatment in Group A and Group B as compared with those before treatment ( P < 0.01) . The exper imental results showed that the difference of cAMP/cGMP ratio between high-dose Lishi Xiaochuan I group and aminophylline group was significant ( P < 0.01) , and the thickness of pulmonary capillary wall in asthmatic guinea pigs was decreased. No collagenous fibers were found. [Conclusion] Lishi Xiaochuan I can increase the ratio of cAMP/cGMP in asthmatic children and asthmatic guinea pigs and reduce the inflammatory reaction in lung tissues of asthmatic guinea pigs.

关 键 词：哮喘 黎氏哮喘Ⅰ号 中医药疗法 超微结构 腺苷环一磷酸 鸟苷环一磷酸 血液 儿童 豚鼠 

分 类 号：R256.12[医药卫生—中医内科学] R285.5[医药卫生—中医学]