HPLC测定视衰康复灵片中大黄素的含量  

Determination of Emodin in Shishuaikangfuling Tablets by HPLC

作　　者：张雯洁[1] 张赟华[1] 李忠琼[1] 金永清[1] 张斌[2] 

机构地区：[1]云南省药品检验所,昆明650011 [2]成都军区民族医药研究所,昆明650034

出　　处：《药物分析杂志》2001年第6期407-409,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定视衰康复灵片中大黄素的含量测定方法。方法:C18柱,甲醇-水-冰醋酸（78:22:0.35）为流动相,流速为1mL·min-1,UV检测波长为427nm。结果:此方法线性关系良好,平均加样回收率为100.5％（n=6）。结论:本方法分离良好,结果准确可靠,为控制视衰康复灵片的质量提供了依据。Objective: To establish the determination method of emodin in Shishuaikangfuling tablets. Method: E-modin in Shishuaikangfuling tablets was extracted by ultrasonic extraction and determined by HPLC on C18 column, with methanol - water- acetic acid glacial (78:22:0.35) solution as a mobile phase, flow rate was 1 mL·min-1 , detection wavelength at 427 nm. Results: This method showed a good linear relationship. The average recovery was 100.5% ( n = 6 ). Conclusion: This method showed good separation and reliable. It may be used for quality control ofShishuaikangfuling tablets.

关 键 词：高效液相色谱 大黄素 视衰康复灵片 含量测定 

分 类 号：R287.81[医药卫生—中药学] R286.0[医药卫生—中医学]