高效液相色谱-蒸发光散射检测法测定氨基糖苷类抗生素中硫酸盐含量的研究  

Research on Determination of Sulfate in Aminoglycoside Antibiotics by HPLC - ELSD

作　　者：刘浩[1] 潘颖[1] 仇仕林[1] 王红武[2] 

机构地区：[1]上海市药品检验所,上海200233 [2]华东理工大学,上海200237

出　　处：《药物分析杂志》2001年第6期429-434,共6页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱-蒸发光散射检测法测定氨基糖苷类抗生素中硫酸盐的含量。方法:采用ODS柱,以含0.015mol·L-1正已胺和0.025mol·L-1甲酸的水-乙腈（88:12）为流动相,流速为10mL·min-1,蒸发光散射检测,检测器温度:45℃,雾化气体（空气）压力:0.35 MPa。结果:SO42-在22～435μg·mL-1范围内呈良好的线性关系,r=0.9997;平均回收率为99.8％,RSD=0.31％（n=6）;日内精密度（RSD=1.1％,n=8）良好。结论:本方法简便,快速,结果准确,可靠,重现性好。Objective: To establish HPLC - ELSD method for the determination of sulfate in aminoglycoside antibiotics . Methods: The column: Shimpack CLC - ODS column (150 mm × 6.0 mm, 5μm) . The mobile phase: water con-taining 0.015 mol·L-1 hexylamine and 0.025 mol·L-1 formic acid- acetonitrile (88:12) . Evaporative light- scattering detection, the detector temperature: 45℃ . The pressure of nebulizing gas (air) : 0.35 MPa. Results: The standard curve was rectilinear in the range of 22-435μg·mL-1, r = 0.999 7; The average recovery was 99.8 % ( RSD = 0.31%, n = 6); The precision in a day (RSD =1.1%, n = 8) was good. Conclusion: The method is simple and rapid, the results are accurate and reproducible.

关 键 词：氨基糖苷类抗生素 硫酸盐 含量测定 高效液相色谱 蒸发光散射检测法 

分 类 号：R978.12[医药卫生—药品] R927.2[医药卫生—药学]