重金属对植物离体组织和细胞生长分化的影响  

The Effects of Heavy Metals on Growth and Differentiation of Plant Cell and Tissue in Vitro.

作　　者：蒋婉茹 杨居荣[2] 许嘉琳[2] 王素芬[2] 

机构地区：[1]中国农业科学院作物研究所,北京100081 [2]北京师范大学环境科学研究所,北京100088

出　　处：《农业环境保护》1991年第5期198-202,共5页Agro-Environmental Protection

基　　金：国家自然科学基金

摘　　要：试验采用组织培养技术,以小麦未成熟胚、烟叶组织及胡萝卜无性细胞系为材料,研究其在含不同浓度的重金属培养基中生长和分化的影响。结果发现:烟叶组织对几种重金属的富集能力不同,其顺序为Cu^(2+)>Cd^(2+)>As^(3+)>As^(5+)>Pb^(2+),而它们对于烟叶组织的毒性效应依次为As^(3+)>As^(5+)>Cu^(2+)>Cd^(2+)>Pb^(2+)。由此看出,对离体组织毒害的强弱并不完全取决于累积数量。In order to conduct further research on the mechanism of the poisons of heavy metals (Cu, As, Cd, Pb)to plant tissue and plant cell, tissue culture method was used in the experiment. Immature embryos of wheat, leaf tissue of tobacco and somaclonal cell of carrot were incubated in the media containing various concentrations of heavy metals. It has been found that the accumulative amounts of heavy metals in the leaf tissue of tobacco is different. The order of accumulative amount is as following, Cu^(2+)>Cd^(2+)>As^(3+)>As^(5+)>Pb^(2+). But the order of poisonous effect to leaf tissue in tobacco is As^(3+)>As^(5+)>Cu^(2+)>Cd^(2+)>Pb^(2+). These facts indicate that the poisonous extent does not depend on the accumulative amounts of heavy metals.In addition, restorative ability or plant tissue sue which was suffered in heavy metals poisonhas also been preliminarily tested.

关 键 词：重金属 植物 离体组织 细胞生长 

分 类 号：X503.23[环境科学与工程—环境工程]