雷公藤内酯醇对人肾小管上皮细胞抗原呈递功能的影响  被引量:27

Triptolide down-regulates induced antigen-presenting cell function of human proximal tubular epithelial cells

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作  者:李恒 刘志红[2] 戴春笋[2] 刘栋[2] 黎磊石[2] 

机构地区:[1]南京大学医学院临床学院 [2]南京军区南京总医院解放军肾脏病研究所,南京210002

出  处:《肾脏病与透析肾移植杂志》2001年第4期303-308,共6页Chinese Journal of Nephrology,Dialysis & Transplantation

摘  要:目的 :观察雷公藤内酯醇对炎症因子刺激下人肾近端小管上皮细胞 (HKC细胞 )抗原呈递能力和共刺激分子表达的影响。  方法 :以IFN γ(2 0 0 μg/L)和TNF α(2 0 μg/L)联合刺激HKC细胞或同时加入不同浓度的雷公藤内酯醇 ,采用流式细胞仪检测细胞表面主要组织相容性二类复合体 (MHC Ⅱ )、共刺激分子B7 1、B7 2及细胞间粘附分子 1(ICAM 1)表达情况。细胞内ICAM 1mRNA表达量采用半定量逆转录PCR法检测。  结果 :普通培养时HKC细胞低度表达MHC Ⅱ分子 ,大量表达ICAM 1分子 ,不表达B7 1和B7 2分子。IFN γ(2 0 0 μg/L)和TNF α(2 0 μg/L)联合刺激 2 4h后 ,小管细胞表面MHC Ⅱ ,ICAM 1,B7分子表达均显著增强 ,其中MHC Ⅱ变化最大。RT PCR显示ICAM 1mRNA表达也明显增加。雷公藤内酯醇可以剂量依赖性地抑制炎症因子引起的细胞MHC Ⅱ ,B7 1以及B7 2分子表达上调 ,但对刺激引起的ICAM 1蛋白及mRNA表达上调无明显影响。  结论 :雷公藤内酯醇可以抑制炎症因子刺激下人肾近端小管上皮细胞MHC Ⅱ及B7分子表达上调 。Objective:To investigate the effect of triptolide on the antigen presenting cell(APC)function of human proximal tubular epithelial cells(HKC cells). Methodology:Class Ⅱ major histocompatibility complex(MHC Ⅱ),B7 1,B7 2 and intercellular adhesion molecule 1(ICAM 1)were detected by flow cytometry in HKC cells exposed to both IFN γ(200 μg/L) and TNF α(20 μg/L)and cultured in media containing different concentration of triptolide for 24 hours.Intracellular ICAM 1 mRNA levels were measured by semi quantitative RT PCR method. Results:Intensive expression of ICAM 1 was found in unstimulated HKC cells,while only low level of MHC Ⅱ expression and almost non expression of B7 1 and B7 2 could be detected in unstimulated HKC cells.With the costimulation of IFN γ and TNF α,the expressions of MHC Ⅱ,B7 1,B7 2 and ICAM 1 were found significantly increased in HKC cells.The induced expressions of MHC Ⅱ,B7 1 and B7 2 were found significantly inhibited by adding triptolide into the media besides IFN γ and TNF α,while the expression of ICAM 1 was found not being affected. Conclusion:Triptolide can effectively inhibit the induced expression of MHC Ⅱ,B7 1 and B7 2 in human proximal tubular epithelial cells.It may influence the APC function and T cell activating ability of tubular cells in inflammatory injuries.

关 键 词:雷公藤内酯醇 肾近端小管上皮细胞 抗原呈递细胞 肾小管间质损伤 HKC细胞 

分 类 号:R692.6[医药卫生—泌尿科学]

 

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