中药材厚朴的DNA扩增产物指纹分析研究  

Studies on DNA Amplification Fingerprinting of Cortex Magnoliae Officinalis

作　　者：王艇[1] 苏应娟[1] 朱建明[1] 李雪雁[1] 曾庆文[2] 夏念和[2] 

机构地区：[1]中山大学生命科学院,广州510275 [2]中国科学院华南植物研究所,广州510520

出　　处：《中药材》2001年第10期710-715,共6页Journal of Chinese Medicinal Materials

摘　　要：采用DNA扩增产物指纹分析(DNA amplification fingerprinting,DAF)技术鉴别中药材厚朴、凹叶厚朴及其常见的伪品、混淆品,获得了清晰可靠的DNA指纹图谱。根据聚丙烯酰胺凝胶上显示的DNA带型差异可简便地区分出厚朴、凹叶厚朴和其它伪品、混淆品。证明DNF技术可以准确、快速地鉴别出中药材厚朴及其伪、混品,在正确引种方面具有较高的价值。Total genomic DNA samples of Chinese traditional medicine(CTM) Cortex Magnoliae Officinalis ('Houpo') ,its counterfeits and substitutes were amplified by DNA amplification fingerprinting (DAT) .Their amplified DNA banding patterns were distinguished on PAGE.The results show that DAF can be used to identify CTM 'Houpo',its counterfeits and substitutes, which is also quite valuable for correctly introducing original plant.

关 键 词：厚朴 DAF 伪品 混淆品 中药材 DNA扩增产物 指纹分析 

分 类 号：R282.5[医药卫生—中药学] R282.71[医药卫生—中医学]