反相高效液相色谱法测定固本益肠片中芍药苷的含量  

Determination of Paeoniflorin in Gubenyichang Tablet by RP-HPLC

作　　者：杨松[1] 靳宝峰[1] 孙玫[2] 邓晓梅[2] 

机构地区：[1]沈阳军区联勤部药品检验所,辽宁沈阳110026 [2]沈阳康乐药厂,辽宁沈阳110015

出　　处：《中成药》2001年第11期794-795,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :建立固本益肠片中芍药苷的含量测定方法。方法 :采用RP HPLC法测定固本益肠片中芍药苷的含量 ,采用C18柱 ,以甲醇∶水 ( 3 2∶68)为流动相 ,检测波长 2 3 0nm。结果 :平均加样回收率为 98.9% ,RSD为 1.2 2 %。结论 :本法简便、灵敏、准确、专属性强 ,具有一定的实用性。Objective:To establish the determination of paeoniflorin in Gubenyichang Tablet.Methods:RP-HPLC method was used for determination of paeoniflorin in Gubenyichang Tablet on C 18 column. The mobile phase was consisted of methanol-water (32∶68) and detection wavelength was at 230nm.Results:The average recovery was 98.9% and RSD was 1.22%. Conclusion:The method is simple, accurate and with good speciality.

关 键 词：芍药苷 固本益肠片 RP^-HPLC 中药 反相高效液相色谱法 含量 

分 类 号：R286.0[医药卫生—中药学]