RP-HPLC法同时测定小果博落回中血根碱和白屈菜红碱的含量  

Simultaneous Determination of Sanguinarine and Chelerythrine in Macleaya Microcarpa(Maxim) Fedde by RP-HPLC

作　　者：安彩贤[1] 杨广德[1] 叶建涛[1] 符军梅[1] 

机构地区：[1]西安交通大学药学院,710061

出　　处：《中成药》2001年第11期824-825,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :测定小果博落回中血根碱和白屈菜红碱的含量。方法 :反相高效液相色谱法。色谱柱 :KromasilC18柱 ,流动相 :乙腈 0 .1%磷酸水溶液 ( 2 5∶75 ) ,检测波长 :2 70nm ,流速 1.0ml·min-1。结果 :白屈菜红碱、血根碱的回收率分别为90 .2 % ,92 .8% ;RSD分别为 1.5 % ,2 .4 %。结论 :为测定小果博落回中生物碱含量提供了准确、灵敏、可靠的分析方法。Objective: To determine the content of sanguinarine and chelerythrine in Macleaya microcarpa(Maxim) Fedde. Methods: RP-HPLC was adopted, kromasil C 18 column (150mm×4.6mm.5μm) with a mobile phase acetonitrile-0.1%(V/V) phosphoric acid solution(25∶75) by UV detector at 270nm. Results: The average recoveries of chelerythrine and sanguinarine were 90.2%,92.8%,RSD were 1.5%,2.4% respectively. Conclusion: A simple, rapid and sensitive method with good precision was established.

关 键 词：高效液相色谱法 小果博落回 血根碱 白屈菜红碱 含量 中药 RP-HPLC 

分 类 号：R286.0[医药卫生—中药学]