差示紫外分光光度法测定尿中诺氟沙星的排泄率  

Determination of the excretion rate of norfloxacin in urine by difference spectrophotometry

作　　者：李好枝[1] 仇峰[1] 董英[2] 孙沂[1] 罗先庆[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳市药品检验所,辽宁沈阳110004

出　　处：《沈阳药科大学学报》2001年第5期332-334,共3页Journal of Shenyang Pharmaceutical University

摘　　要：采用差示紫外分光光度法测定了尿中诺氟沙星的排泄率。尿样分别用HCl(0 0 2mol/L)和NaOH(0 0 2mol/L)稀释成相同浓度 ,以前者为样品液 ,以后者为参比液 ,在波长 2 85nm测定吸收度差值 (ΔA) ,回归方程为ΔA =0 0 4 47C +0 0 80 8(r =0 9971 ) ,诺氟沙星尿药浓度在 2～1 2 μg/mL呈线性 ,平均回收率为 97 6% (RSD =4 7% ,n =6) ,日内RSD小于 1 9% (n =6) ,日间RSD小于 4 2 % (n =6)。 4例受试者的尿药排泄率为 3 0 4 9%～ 3 1 96%A difference spectrophotometry for determination of norfloxacin in urine was developed. Two solutions with the same concentrations were added to 0 02 mol/L HCl and 0 02 mol/L NaOH separately ,the former was used as the sample and the latter as the reference for determination of Δ A value. The wavelength of maximum absorption was 285 nm.The calibration curve of norfloxacin was linear in the range of 2～12 μg/mL ( r =0 997 1),the average recovery of norfloxacin was 97 6%(RSD=4 7%, n =6).The day to day and within day relative standard deviations were less than 1 9% ( n =6)and 4 2%( n =6) respectively. The excretion rate of the norfloxacin in urine for four healthy men was 30 49%～31 96%.

关 键 词：诺氟沙星 差示紫外分光光度法 尿药排泄率 氟哌酸 

分 类 号：R975[医药卫生—药品] R969.1[医药卫生—药学]