小巨核细胞免疫酶标染色在鉴别骨髓增生异常综合征和巨幼细胞贫血中的意义  

THE SIGNIFICANCE OF MICROMEGAKARY OCYTE ENZYME LINKED IMMUNOSORBENT ASSAY IN DIFFERENTIATING MYELODYSPLASTIC SYNDROME FROM MEGALOBLASTIC ANEMIA

作　　者：桑玉旗 赵锐利 

机构地区：[1]菏泽市市立医院内科,山东省菏泽市274003

出　　处：《齐鲁医学杂志》2001年第4期276-278,共3页Medical Journal of Qilu

摘　　要：①目的　探讨小巨核细胞免疫酶标染色在鉴别骨髓增生异常综合征 (MDS)和巨幼细胞贫血 (MegA)中的意义。②方法　采用碱性磷酸酶 抗碱性磷酸酶桥联免疫酶标 (APAAP)法 ,对 3 0例MDS和 3 4例MegA病人骨髓涂片染色 ,观察巨核细胞阳性率并计数 ,并与 3 3例正常骨髓进行比较。③结果　小巨核细胞在MDS中的阳性率为93 .3 % ,在MegA中的阳性率为 2 3 .8% ,差异有极显著意义 ( χ2 =3 1.5 6,P <0 .0 1) ;二者均明显高于正常对照组( 12 .3 % ) ,差异有显著性 ( χ2 =4 8.94 ,13 .69,P <0 .0 1)。MDS中小巨核细胞数量为每片 3 4个 ,MegA中小巨核细胞数量为每片 4 .8个 ,两者比较 ,差异有显著性 (u =78.2 4 ,P <0 .0 1) ;两组与正常对照组 (每片 0 .3个 )比较 ,差异均有极显著性 (u =648.5 6,4 3 2 .78,P <0 .0 1)。MDS中的小巨核细胞以淋巴样小巨核细胞、单圆核小巨核细胞为主 ;MegA中的小巨核细胞为单圆核、多核小巨核细胞 ,未见淋巴样小巨核细胞。Objective To investigate the efficacy of micromegakaryocyte enzyme linked immunosorbent assay(MELIA) in differenti ating myelodysplastic syndrome (MDS)from megaloblastic anemia(MegA). Methods The marrow smears of patients with MDS( n =30)and MegA( n =34) and healthy volunteers( n =33) were stained by APAAP tec hnique. The megakaryocytes of each marrow smear were counted and cla ssified according to morphologic chracteristics and compared with normal mar row smears. Results The positivity rate of micromegakaryocytes of patie nts with MDS was significantly higher than that of the patients wit h MegA(93.3% vs 23.8%, χ 2=31.56, P < 0.01). The rates of the both g roups were significantly higher than that of healthy volunteers(93.3%,23. 8% vs 12.3%, χ 2=48.94, 13.69, P < 0.01). The average number of micr omegakaryocytes in each marrow smear of the patients with MDS was mor e than that of the patients with MegA (34/each smear vs 4.8/each smea r, u=78.24,P <0.01, both were more than that of healthy volunteers(34/ea ch smear , 4.8/each smear vs 0.3/each smear, u= 648.56 ,432.78,P <0.01, respectively). The micromegakaryoctes of the patients with MDS mainly in cluded lymphoid or mono round nuclear micromegakaryocytes, but that of the patients with MegA included mono round or multi nuclear micromega karyaytes, and no evidence of lymphoid micromegakaryocyte was found. Conclusion MELIA technique is of great significance in different iating myelodysplastic sydrome from megaloblastic anemia. [WTHZ

关 键 词：小巨核细胞 免疫酶技术 骨髓增生异常综合征 染色 巨幼细胞贫血 

分 类 号：R551.3[医药卫生—血液循环系统疾病] R556.2[医药卫生—内科学]