犬冠状病毒YS1株细胞培养弱毒的实验免疫研究  

Experimental immunization of attenuated canine coronavirus strain YS1

作　　者：余春[1] 夏咸柱[1] 范泉水[2] 何洪彬[3] 邱薇[2] 黄耕[1] 王允海[4] 

机构地区：[1]解放军军需大学军事兽医研究所,吉林长春130062 [2]成都军区医学研究所,云南昆明650032 [3]东北农业大学生命科学院,黑龙江哈尔滨1500302 [4]黑龙江省军区军犬训练队,黑龙江哈尔滨150052

出　　处：《畜牧与兽医》2001年第4期1-4,共4页Animal Husbandry & Veterinary Medicine

基　　金："九五"全军医药卫生重点基金资助项目部分内容( 98Z0 85 )

摘　　要：应用纯化后的犬冠状病毒 (CCV)YS1株细胞培养致弱的弱毒 (CCVYS1V60 ) ,经口鼻和肌肉接种CCV ,SN抗体小于 1∶2的易感犬作安全试验 ,结果未见任何CCV临床症状与病理解剖学改变 ;用不同剂量的该CCV弱毒分组免疫CCV易感犬 ,经SN抗体测定与用CCV强毒攻毒试验 ,结果SN抗体达 1∶60以上的犬 90 %以上可获得免疫保护 ;应用该CCV弱毒分别免疫母源抗体达 1∶4和 1∶8的 2组试验犬 ,第 1次免疫 1 4d后SN抗体均未见升高 ,追加 2～ 3次免疫后 ,SN抗体才逐渐上升至 1∶60以上的免疫保护水平 ;用CCV易感犬和猫肾传代细胞 (CRFK)分别将该CCV细胞培养弱毒连续传 5代和 2 0代 ,结果对犬仍然安全 ,免疫原性也未见下降 ;与犬瘟热病毒 (CDV)、犬传染性肝炎病毒 (ICHV)和犬细小病毒(CPV)弱毒作免疫互扰试验 ,结果与各弱毒的单独免疫结果未见差异 ;该毒的免疫期在 1年以上 ,- 2 0℃冻结保存的保存期为 9个月。Plaque purified canine coronavirus attenuated strain YS1(CCV YS1 V 60 )was employed to inoculate pups with SN titer of 1∶2 in order to test its safety.The result indicated that pups didnt show any clinical symptoms and no pathological change was found.When different doses of this virus strain were then used to immunize dogs,more than 90% immunized dogs with SN antibody titer 1∶60 were protected from virulent virus challenge.The SN antibody titer of dogs with a maternal antibody of 1∶4 to 1∶8 did not rise after the first inoculation,while it rose to the protection level of 1∶60 after the second and third times of immunization.CCV YS1 V 60 was still stable and kept its immunogenicity after 5 continuous passages in dogs and 20 continuous passages on CRFK,respectively.Mixed immunization trial between CCV YS1 V 60 and CDV?ICHV and CPV didnt show interference.Immunity duration for dogs by CCV YS1C 60 lasted for more than 1 year,and CCV YS1V 60 could be frozen and stored at -20℃ for 9 months.

关 键 词：安全性 免疫原性 遗传稳定性 大病 大冠状病毒 YS1株细胞 弱毒 

分 类 号：S858.292.5[农业科学—临床兽医学]