抗J亚群禽白血病病毒囊膜糖蛋白特异性单克隆抗体的研制及其特性  

DEVELOPMENT AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO SUBGROUP J AVIAN LEUKOSIS VIRUS

作　　者：秦爱建[1] 崔治中[1] LEE LUCY FADLY ALY 

机构地区：[1]扬州大学动物医学系, [2]Avian Disease and Oncology Laboratory,USDA,East Lansing,MI48823USA

出　　处：《畜牧兽医学报》2001年第6期556-562,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省教委留学回国基金 ;扬州大学博士启动基金

摘　　要：:J亚群禽白血病病毒 (ALV -J)是一种主要感染肉用型鸡的反转录病毒。本研究用表达ALV -J囊膜蛋白基因产物的Sf9细胞免疫Balb/c小鼠 ,取其脾脏细胞与骨髓瘤细胞NS1进行融合 ,获得了 4株特异性抗ALV -J的单克隆抗体。免疫荧光分析结果表明 ,3株单克隆抗体仅与所试验的ALV -J毒株反应 ,而不能与ALV的A、B、C、D和E亚群的毒株反应。有趣的是 ,有一株单克隆抗体可以与所有试验的外源性ALV毒株反应 ,但不与内源性的E亚群反应。WesternBlot和免疫沉淀试验结果表明 ,单克隆抗体识别的ALV -J囊膜糖蛋白的分子量为 90 - 94kD ,识别未糖基化的囊膜蛋白分子量约为 5 3kD。用这些单克隆抗体能检测出ALV -J病毒感染鸡胚成纤维细胞中的病毒抗原。这些结果提示这些单克隆抗体可用于ALVAvian leukosis virus subgroup J (ALV-J) is a retrovirus which infects meat-type chickens.A panel of monoclonal antibodies (Mabs) to ALV-J was developed by fusion between NS1 and spleen cells from mice immunized with Sf9 cells infected with recombinant baculovirus Hcl-env.Using immunofluorescence assay (IFA),three of four Mabs reacted with ALV-J,but not with subgroups A,B,C,D or E of ALV.Interestingly,one of the four Mabs reacted with all exogenous subgroups of ALV but not with endogenous subgroup E.Western Blot and immunoprecipitation tests showed that molecular weight of ALV-J envelope glycoprotein or unglycosylated envelope protein recognized by Mabs was about 90 kD and 53 kD respectively.Using these Mabs we can detect the ALV-J antigen in CEF infected with ADOL-Hcl.These Mabs can be used for diagnosis and epidemology of ALV-J.

关 键 词：禽白血病病毒 J亚群 囊膜蛋白 单克隆抗体 囊膜糖蛋白 

分 类 号：S852.65[农业科学—基础兽医学] S855.3[农业科学—兽医学]