皱纹盘鲍肌动蛋白基因启动子的克隆和序列分析  

Cloning and sequence analysis of actin gene promoter in Haliotis discus hannai

作　　者：张志峰[1] 茅云翔[1] 潘洁[1] 汪小龙[1] 包振民[1] 

机构地区：[1]青岛海洋大学海洋生命学院,山东青岛266003

出　　处：《水产学报》2001年第5期398-401,共4页Journal of Fisheries of China

基　　金：国家863青年基金项目(863-819-Q-05)

摘　　要：从皱纹盘鲍雌性个体的足部肌肉提取总ＤＮＡ后，通过聚合酶连式反应（ＰＣＲ）技术扩增得到一个扩增产 物。经克隆、筛选、确定重组子产物。测序得到了长度为５１１ｂｐ的启动子片段。分析测序结果发现，皱纹盘鲍 肌动蛋白基因启动子ＤＮＡ序列与目前已知的红鲍相应序列的相似度为９５％；ＯＣ碱基含量为３８．９３％，较红 鲍的低（５９．２％）；所得序列含有高度保守的基本表达调控元件，即一个ＣＡＡＴ框和四个：ＴＡＴＡ框。Total DNA from foot muscle of female individial in Haliotis discus hannai was extracted by routine method. Actin gene promoter was amplified by PCR technique. A recombinant was obtained after cloning and screening. Its length is 511 bp. The result shows after the sequence is analysed: The homologous series of nuleotides sequence between Haliotis discus hannai and Haliotis rufescens is 95%. The CC base content of Haliotis discus hannai is 38. 93%. This value is 59. 2% lower than that of Haliotis rufescens. The sequence includes expression of control elements whose high level of conservation can be seen, i. e. four TATA boxes and one CAAT box.

关 键 词：皱纹盘鲍 肌动蛋白基因 启动子 序列分析 克隆 

分 类 号：Q959.212[生物学—动物学]