乙肝表面抗原a表位粘膜疫苗的构建及免疫原性检测  

CONSTRUCTION OF MUCOSAL VACCINE DERIVED FROM HBV SURFACE ANTIGEN EPITOPE A AND THE ASSAY OF ITS IMMUNOGENICITY

作　　者：刘相国[1] 杨恭[1] 邱并生[1] 田波[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2001年第5期625-629,共5页Acta Microbiologica Sinica

摘　　要：利用DNA重组技术 ,构建霍乱毒素B亚单位与乙肝病毒表面抗原a表位的融合基因 ,并在大肠杆菌表达体系pET 30a BL2 1 (DE3)中获得以包涵体形式的高效表达。免疫印迹表明表达产物具有免疫学活性 ,包涵体复性后 ,表达产物能够象CTB那样形成五体 ,以腹腔注射、灌胃。The fusion gene of HBV(adr) surface antigen epitope and B subunit of Cholera toxin was constructed and expressed successfully in E.coli at high yield. After denaturation\|renaturation process, SDS\|PAGE analysis showed that most of the renatured product reassociated in a pentameric form which was the same as natural CTB. Western blot analysis indicated that the immunogenicity of HBsAg antigen epitope was conserved. Moreover, ELISA analysis of the sera of orally, intranasally and intraperitoneally immunized mice showed that the circulating IgG antibodies to HBsAg were developed. The results may be helpful for constructing the novel mucosal vaccine with high efficacy.

关 键 词：CTB 乙肝病毒表面抗原a表位 融合蛋白 ELISA 粘膜免疫疫苗 免疫原性 

分 类 号：R186[医药卫生—流行病学]