家蚕AFLP连锁框架图谱的构建  

Construction of AFLP linkage map of the silkworm (Bombyx mori)

作　　者：朱玉芳[1,2] 谭远德[2,3] 万春玲[2] 鲁成[4] 贺一原[5] 周泽扬[4] 向仲怀[4] 

机构地区：[1]苏州大学生命科学学院生物科学系 [2]南京师范大学生命科学学院,南京210097 [3]湖南师范大学生命科学学院,长沙410081 [4]农业部重点实验室,西南农业大学蚕丝学院,蚕业科学研究所 [5]中国农业科学院蚕业科学研究所

出　　处：《昆虫学报》2001年第4期483-493,共11页Acta Entomologica Sinica

基　　金：江苏省科委 (A1078);江苏省教委自然科学基金课题 (2 10 75 ) ;国家自然科学基金项目 (39870 5 6 8);国家自然科学基金重点项目 (3970 730 )

摘　　要：利用改进的AFLP分子标记方法 ,对家蚕Bombyxmori回交一代BC1群体进行连锁图谱的构建。经 17组AFLP引物的选择性扩增 ,共得到 4 30个多态位点 ,卡方检验后有 2 5 3个为有效位点。利用Mapmaker Exp (Version 3 0b)软件作连锁分析 ,其中 16 3个标记分属 2 8个连锁群 ,连锁群标记数变化范围是 2～ 2 8个 ,平均每个连锁群标记数为 5 8个 ,该图覆盖的基因组长度为 2 998 9cM(图距单位 ) ,连锁群长度变化范围为 4 5～ 6 5 2 8cM ,连锁群的平均长度为 10 7 1cM ,平均图距为4 5～ 36 7cM。AFLP markers were mapped using BC 1 population [od 100 ×(782×od 100 )]. From 47 BC 1 samples 430 polymorphic markers were detected using an improved method of AFLP, called silver staining AFLP, which was to some extent different from the original AFLP method, called radiation AFLP. In this study 17 AFLP primer pairs and two restriction enzymes PstⅠ and TaqⅠ were used. Being tested by chi-square test under P=0 05 level for 1∶1 segregation proportion, 253 valid loci that might be used in mapping were obtained. The ratio of the valid loci to polymorphic loci was 58 84%. Twenty eight linkage groups with 163 loci were mapped by using Mapmaker/Exp(Version 3 0b), in which we set LOD=3 0 and maximum recombination fraction= 0 3, and Kosambi function. There were 90 unlinked loci in these 253 valid loci and the ratio of mapping loci to valid loci was 64 43%. The number of loci mapped in the 28 groups was at a range of 2～28 and there were 5 8 (average value) loci per group. The total length of the groups was 2 998 9 cM. The lengths of the 28 groups varied at a range of 4 5～652 8 cM and the average distances between loci on the 28 groups were at a range of 4 5～36 7 cM. The average length of groups was 107 1 cM.

关 键 词：家蚕 分子标记 AFLP 连锁图谱 

分 类 号：S881.26[农业科学—特种经济动物饲养]