类产碱假单胞菌酪氨酸酶基因重组子所产黑色素性质的研究  

MELANIN PROPERTIES STUDIES OF THE RECOMBINANT OF PSEUDOMONAS PSEUDOALCALIGENES CONTAINING TYROSINASE GENE

作　　者：蔡信之[1] 

机构地区：[1]盐城师范学院生物系,盐城224002

出　　处：《微生物学报》2001年第6期699-703,共5页Acta Microbiologica Sinica

基　　金：江苏省盐城市科学基金资助项目 (S981 1 )

摘　　要：将构建的含有嗜麦芽假单胞菌酪氨酸酶基因的重组质粒导入从自然病死蝇蛆体内分离的具有显著杀蛆作用的类产碱假单胞菌体内 ,使后者获得了稳定产生黑色素的能力 ,具有很强的抗辐射作用。所产黑色素是无定形物质 ,在大多数有机和无机溶剂中溶解度低或不溶解 ,在碱性溶液中有较好的溶解性 ,在中性及弱酸性溶液中溶解度低 ,在pH <4的溶液中沉淀 ;可用过氧化氢或次氯酸钠氧化 ,也能被银离子或硫化氢还原 ;含有自由基 ,并能清除由紫外线诱导产生的自由基 ;能吸收所有波长的光线 ,在紫外波长下吸收值最大 ,能有效地保护紫外辐射对蛋白质及DNA等生物大分子物质的损害。Pseudomonas pseudoalcaligenes is a notably killing maggots bacterium, which was isolated from natural dead maggots.Tyrosinase gene of P.maltophilia AT18 has been introduced into P.pseudoalcaligenes, and enabled it the ability of producing melanin steadily. Antiradiation effect of the melanin is quite strong. The melanin of the recombinant is nonfixiform material.It's solubility is very little in many sorts of organic or inorgonic solvent. It's solubility is big in alkaline solution, but little in neutral or weak acid solution. It is deposited when pH<4. It is oxidized by H-2O-2 or NaC10. It is also reduced by Ag++ or H-2S. It has free radical and it can absorbs free radical generated by ultra-violent ray. It can absorbs ray of all sorts of wave length. The absorption rate of ultra-violet ray is the biggest in all sorts of wave length. It can effectively protects protein, DNA and other biomacromolecule matter against the damages by ultra-violet ray.

关 键 词：类产碱假单胞菌 酪氨酸酶基因重组子 黑色素 性质 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]