葡萄糖对重组CHO细胞生长代谢及EPO表达的影响  

Effects of Glucose on Growth, Metabolism and EPO Expression in Recombinant CHO Cell Cultures

作　　者：孙祥明[1] 张元兴[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物工程学报》2001年第6期698-702,共5页Chinese Journal of Biotechnology

摘　　要：在CHO细胞批培养中 ,葡萄糖浓度从 8 9增加到 4 9 6mmol L ,最大活细胞密度没有明显的差异 ,乳酸对葡萄糖的得率系数首先随着葡萄糖浓度的增加而增加 ,葡萄糖浓度达到 17 9mmol L后 ,乳酸对葡萄糖的得率系数基本上维持恒定。在本实验中 ,葡萄糖浓度对谷氨酰胺代谢没有明显的影响。EPO的累积浓度首先随着起始葡萄糖浓度的增加 ( 8 9～ 17.9mmol L)而增加 ,进而又随着葡萄糖浓度的增加 ( 17 9～ 4 9.6mmol L)而下降 ,表明存在一最适浓度 ,在此浓度下重组CHO细胞的EPO表达最大。The maximum viable cell density almost kept constant in the experimental range of initial glucose concentrations from 8.9 to 49.6mmol/L in the batch cultures of recombinant CHO cells. The yield coefficient of lactate to glucose increased with the initial glucose concentrations from 8.9 up to 17.9mmol/L, and then kept constant with above 17.9mmol/L. There was little effect of glucose on glutamine metabolism. It was also demonstrated that the accumulative concentration of EPO increased with the increase of glucose concentration from 8.9 to 17.9mmol/L, and decreased with its further increase from 17.9 to 49.6mmol/L. It is showed that there is an optimum glucose concentration at which the EPO express of cultured reombinant CHO cells is on the peak.

关 键 词：CHO细胞 葡萄糖 代谢 红细胞生成素 动物细胞培养 

分 类 号：Q813[生物学—生物工程]