大鼠部分肝切除前后热激处理对过氧化物酶基因表达的影响  

Expression Difference of the Peroxidase Genes in Partial Hepatectomy and Heat Shock

作　　者：徐存拴 张为民[1] 王莹[2] 赵绪永[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453002 [2]暨南大学生命科学技术学院,广州510632

出　　处：《遗传》2001年第6期535-538,共4页Hereditas（Beijing)

基　　金：河南省生物工程重点实验室资助项目 (编号 :PKL990 0 2 )

摘　　要：用过氧化物酶原位复性电泳 (SDS -POD -PAGE)技术分析了部分肝切除 (partialhepatectomy ,PH)、部分肝切除后再热激 (partialhepatectomyfollowingheatshock ,PH -HS)和先热激再部分肝切除 (heatshockfollowingpartialhepate ctomy ,HS -PH)后肝再生期间过氧化物酶 (POD)基因表达差异 ,结果表明 ,PH中表达的POD基因种类 (7个 ) >PH-HS(5个 ) >HS -PH(3个 ) ;三个模型的POD总活性为 :HS -PH >PH >PH -HS。根据实验结果推测 ,POD在肝再生和肝损伤恢复中起一定作用。Expression difference of the peroxidase (POD) genes during liver regeneration after partial hepatectomy (PH), partial hepatectomy following heat shock (PH-HS) and heat shock following partial hepatectomy (HS-PH) was analyzed by POD renatured electrophoresis (SDS-POD-PAGE). The results showed that the expressed POD gene kinds in PH, PH-HS and HS-PH were 7, 5 and 3 respectively, and that the total activities of POD in the three models were HS-PH > PH > PH-HS. The results suggested that POD play some roles during liver regeneration and in recovery after liver damage.

关 键 词：部分肝切除 热激 过氧化物酶 等位酶 诱导酶 肝再生 基因表达 

分 类 号：Q344.13[生物学—遗传学]