汉坦病毒76-118株与R22株基因重排的实验研究  

Genetic Reassortment Between Hantaan Virus Strains and Seoul Virus Strains

作　　者：康文臻[1] 黄长形[1] 白雪帆[1] 杨为松[1] 

机构地区：[1]第四军医大学唐都医院感染病科,西安710038

出　　处：《中国媒介生物学及控制杂志》2001年第4期289-292,共4页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金资助项目 (编号 :3 96 70 6 5 0 )

摘　　要：目的 :研究汉坦病毒汉滩型与汉城型之间能否发生基因重排 ,以及发生重排的频率和特点。方法 :用汉坦病毒汉滩型 (HTNV) 76 - 118株与汉城型 (SEOV)R2 2株混合感染Vero -E6 细胞 ,空斑形成实验挑取子代克隆株 2 4株 ,传代培养后用PCR方法鉴定。结果 :发现 2 4株子代病毒 (70 .83% )的基因组来自 76 - 118株或R2 2株 ;1株 (4 .17% )HTNV型与SEOV型引物扩增均为阳性 ;1株 (4 .17% )两型病毒引物扩增均为阴性 ;4株(16 .67% )发生了基因片段重排。结论Objective:To determine the frequency and characteristics of reassortment among viruses causing hemorrhagic fever with renal syndrome (HFRS). Methods:Mixed infections were initiated in tissue culture by using Hantaan virus strains (76-118) and Seoul virus strains (R22). Potential reassortant virus plaques were screened by multiplex RT-PCR, using primers specific for individual genome segments of each strain. Results:Most of the progeny virus plaques (70.83% of 24) had the parental genotype of 76-118 strain or R22 strain. 1 of 24 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 16.67% of 24 progeny plaques tested, involving the M and L segments. In addition, approximately 4.17% of the progeny virus plaques appeared to contain M segments originating from both parental virus strains, i.e., they were diploid. Conclusion:Genetic reassortment can occur between Hantaan virus strains and Seoul virus strains.

关 键 词：汉坦病毒 肾综合征出血热 聚合酶链式反应 基因重组 

分 类 号：R373.32[医药卫生—病原生物学] R512.8[医药卫生—基础医学]