长白蝮蛇类凝血酶基因的克隆及分析(英文)  

Cloning of cDNA for Thrombin-like Enzyme from Agkistrodon halys Ussuriensis Snake Venom

作　　者：杜道林[1] 邓晓东[2] 吴文芳[3] 吕国安[3] 徐梅[3] 

机构地区：[1]中国科学院沈阳应用生态研究所,中国辽宁沈阳110016 [2]热带作物生物技术国家重点实验室,中国海南海口571101 [3]海南师范学院生物系,中国海南海口571158

出　　处：《生命科学研究》2001年第3期230-237,共8页Life Science Research

基　　金：中国科学院"九五"重点项目 ( KZ-95 2 -SI-2 2 6)~~

摘　　要：从长白蝮蛇 (Agkistrodon halys Ussuriensis)毒腺中抽提总 RNA,采用 RT-PCR扩增其类凝血酶基因 ,经全序列测定 ,类凝血酶基因 Ussurin全长为 70 8个核苷酸 ,即编码 2 3 6个氨基酸 ;根据同源性 ,推测它的活性中心为 His43,Asp88和 Ser182 ;二硫键为 Cys7-Cys14 1,Cys2 8-Cys44,Cys76 -Cys2 34 ,Cys12 0 -Cys188,Cys152 -Cys16 7和 Cys178-Cys2 0 3.该蛇毒类凝血酶 c DNA序列及推导的氨基酸序列均为首次报道 .Total RNA were extracted from the venom gland of the Agkistrodon halys Ussuriensis snake. A thrombin like enzyme gene was amplified by RT PCR, cloned, and its nucleotide sequence was determined. The enzyme called ussurin cDNA encodes 708 nucleotides, namely 236 amino acids. Based on homo1ogy, the catalytic residues and disulfide bridges of ussurin were deduced as follows: catalytic residues, His 43 , Asp 88 and Ser 182 ; and disulfide bridges, Cys 7 Cys 141 , Cys 28 Cys 44 , Cys 76 Cys 234 , Cys 120 Cys 188 , Cys 152 Cys 167 and Cys 178 Cys 203 .

关 键 词：长白蝮蛇 类凝血酶 基因克隆 序列分析 蛇毒 

分 类 号：Q959.62[生物学—动物学] R996.3[医药卫生—毒理学]