反相高效液相色谱法测定地红霉素的含量  

Determination of dirithromycin by RP-HPLC

作　　者：关荣才[1] 周志凌[1] 

机构地区：[1]安徽省药品检验所,合肥230061

出　　处：《安徽医药》2001年第3期219-221,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：报道反相高效液相色谱法测定地红霉素含量的方法。采用ODS柱 (15 0× 4 6mm) ,以磷酸三乙胺缓冲液 乙腈 甲醇 (35∶45∶2 0 )为流动相 ,使用紫外检测器 ,于 2 15nm波长处测定。线性范围为 1 0～ 9 0g·L-1(r =0 9999) ,RSD =0 9% (n =9)。检测限为 5 0× 10 -2 mg。本法可对地红霉素中的 (9)S 红霉胺、16 (R) 地红霉素、16 (S) 地红霉素的等组分进行分离测定 ,具有快速、简便、灵敏。RP HPLC method was established for the determination of dirithromycin. Dirithromycin can be separated on ODS column with a mixture of phosphoric acid and triethylamine, acetonitrile and methanol (35∶45∶20) as mobile phase and detected at 215 nm. The flow rate was 1 0 ml·min -1 . The standard curve was linear in the concentration range 1 0 to 9 0 g·L -1 and the correlation coefficient was 0 9999. The relative standard deviation was 0 9%( n= 9). The detection limit was 5 0×10 -2 mg. 9(S) Erythromycylamine, dirithromycin and dirithromycin 16S epimer can be separated and detected. The method is simple, rapid, accurate and reliable.

关 键 词：反相高效液相色谱法 地红霉素 含量测定 药代动力学 

分 类 号：R927.2[医药卫生—药学]