烫伤延迟复苏大鼠组织白细胞介素18mRNA表达的变化  

The dynamic change in the expression of interleukin 18 mRNA in the tissue of scalded rats after delayed resuscitatio n

作　　者：刘京衢[1] 高维谊[1] 姚咏明[1] 柴家科[1] 郭振荣[1] 盛志勇[1] 

机构地区：[1]解放军三0四医院烧伤研究所,北京100037

出　　处：《中华烧伤杂志》2001年第5期286-288,共3页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划资助项目 (G1990 5 42 0 3)

摘　　要：目的　探讨烫伤延迟复苏后不同组织白细胞介素 18(IL - 18)mRNA的动态表达规律。　方法　采用大鼠TBSAⅢ度烫伤延迟复苏模型 ,5 4只大鼠随机分为正常对照组、烫伤延迟复苏组、选择性消化道脱污染 (SDD)预防组 ,分别在伤前及伤后 2、8、16、2 4h ,采用逆转录聚合酶链式反应 ,检测肠、肺、肝、肾等组织IL - 18mRNA含量。　结果　烫伤延迟复苏后体循环内毒素水平显著升高 ,8、2 4h达高峰 (P <0 0 1) ,给予SDD预防治疗可显著降低内毒素峰值 (P <0 0 5 ) ;另一方面 ,烫伤后 2h ,肺、肝、肾等组织IL - 18mRNA含量表达较伤前值有显著升高 ,烫伤后 8h达高峰(P<0 0 1) ,且一直持续至伤后 2 4h ,给予SDD预防后可不同程度抑制IL - 18mRNA的表达 (P <0 0 5～ 0 0 1)。相关分析显示 ,体循环内毒素水平同肠、肺、肝组织IL - 18mRNA呈显著正相关 (r值分别为 0 2 98、0 2 90、0 36 5 ,P <0 0 5～ 0 0 1)。　结论　肠、肺、肝、肾等组织IL - 18mRNA表达在烫伤早期即显著增多 ,并呈逐渐升高的趋势 ,创伤后内毒素血症对机体多种组织IL - 18mRNA基因表达具有重要影响。Objective To explore the dynamic chang e in the expression of interleukin 18 (IL-18) mRNA in the tissue of scalded rat s after delayed resuscitation. Methods Wistar rats inflicted b y 30% TBSAⅢ degree scalding were employed as the model.Fifty-four rat s were randomly divided into normal control (C),scalding and del ayed resuscitation group (SD) and preventive group of selective decontami nation of digestive tract(SDD) group.The RT-PCR was applied to determine the ti ssue contents of IL-18 mRNA in intestine,lungs,liver and kidneys at 2,8,16 and 24 PBHs. Results Plasma level of LPS increased signific antly and reached summit level at 8 and 24 PBHs after delayed resuscitation afte r scalding(P<0.01),but decreased obviously (P<0.05) after the preventive application of SDD.Furthermore,the expression of tissue IL -18 mRNA in lungs,liver and kidneys since 2 PBHs increased evidently when compared to that before scalding and reached peak value at 8 PBH (P<0.01) and lasted up to 24 PBHs.The expression of IL-18 mRNA could be inhibited to va rying degrees by preventive application of SDD (P<0.05～0.01).It was indic a ted by correlative analysis that plasma level of LPS was positively correlated t o the expression of tissue IL-18 mRNA in intestine,lungs and liver (r=0.29 8,0.290 and 0.365 respectively with P<0.05～0.01). Conclusion The expression of tissue IL-18 mRNA in intestine,lungs,liver and kidneys increased significantly during early postburn stage and progressively enhanced thereafter.And the expression oftissue IL-18 mRNA in a variety of body tissue was significantl y affected by post-traumaticendotoxemia.

关 键 词：白细胞介素18 内毒素血症 基因表达 延迟复苏 烫伤 

分 类 号：R644[医药卫生—外科学]