L-选择素介导白细胞-烧伤血清激活的内皮细胞粘附  

The adherence of leukocytes mediated by L-selectin to endothelia activated by burn sera

作　　者：倪俊 刘志国[2] 陈玉林[2] 于宝军[2] 杨红莲 方勇[2] 张素贞[2] 郇京宁[2] 刘训龙 

机构地区：[1]解放军第八十五医院外科,上海200052 [2]长海医院烧伤科 [3]长征医院外科

出　　处：《中华烧伤杂志》2001年第4期234-235,共2页Chinese Journal of Burns

基　　金：上海市医学领先专业基金

摘　　要：目的 研究Ｌ－选择素在大鼠中性粒细胞（ＰＭＮ）与烧伤血清激活的内皮细胞间粘附中的介导作用。方法 用组织块法培养大鼠肺微血管内皮细胞（ＰＭＥＣ），用持续灌注法收集并分离大鼠ＰＭＮ。 收集标本测定Ｌ－选择素单抗、Ｌ－选择素配体硫苷脂孵育的ＰＭＮ及ＰＭＥＣ间粘附率。结果 Ｌ－选择素单抗或硫苷脂能降低中性粒细胞与烧伤血清激活的ＰＭＥＣ间粘附率。结论 Ｌ－选择素介导了ＰＭＮ与被激活的ＰＭＥＣ之间的粘附。Objective To study the mediating effects of L-selectin on the adherence of rat PMNs to the endothelia activated hy burn sera. Methods Pulmonary microvascular endothelial cells ( PMEC) of rat were cultured by tissue block method. Rat PMNs were harvested by continuous perfusion. The samples were collected for the detection of the adhering rate of PMEC to the PMNs after being cultured with L-selectin monoclonal antibody( mAb) , L-selectin ligand and sulfatide, respectively. Results The adhering rate of PMNs to PMEC was obviously lowered by the adding of L-selectin mAb and sulfatide. Conclusion The ad- herence of PMNs to activated PMEC could be mediated by L-selectin.

关 键 词：烧伤 细胞粘附 选择素 内皮细胞 

分 类 号：R459.7[医药卫生—急诊医学]