Phadiatop和sIgE检测在儿童哮喘诊断中的应用  

Application of Phadiatop Test and Specific IgE Test in the Diagnosis of Children with Asthma

作　　者：雷其洪[1] 葛传生[1] 季纯珍[1] 姚劲[1] 赵德育[1] 赵维美[1] 

机构地区：[1]南京医科大学附属南京儿童医院呼吸科,江苏南京210008

出　　处：《中国当代儿科杂志》2001年第5期501-502,共2页Chinese Journal of Contemporary Pediatrics

摘　　要：目的　探讨CAP过敏原检测系统中吸入性过敏原过筛试验 (Phadiatop)检测和特异性IgE(sIgE)检测在小儿哮喘病因诊断中的价值。方法　应用荧光酶联免疫法 ,对 15 3例小儿哮喘、40例支气管肺炎进行Pha diatop ,sIgE和血清总IgE检测。 结果　哮喘患儿Phadiatop和尘螨sIgE阳性率分别为 6 6 .0 1%和 5 9.48% ,而支气管肺炎患儿两者阳性率分别为 12 .5 %和 7.5 % ,两组间差异有显著性 (P <0 .0 5 )。Phadiatop ,sIgE灵敏度分别为0 .6 6和 0 .5 9,特异度分别为 0 .88和 0 .93,两种检测的一致性高。结论　Phadiatop检测是哮喘病因诊断简便、可靠的体外过筛试验 。Objective To explore the value of Phadiatop test and specific IgE test in the diagnosis of children with asthma. Methods Using Flo ELISA, Phadiatop, specific IgE and total IgE of serum were determined in 153 cases with asthma and 40 cases with bronchopneumonia. Results The positive rates of Phadiatop and specific IgE in the asthma group ( 66.01% and 59.48% , respectively) were significantly higher than those in the bronchopneumonia group ( 12.5% and 7.5% , respectively)(P< 0.05 ). Phadiatop test had a sensitivity of 0.66 and a specificity of 0.88 , and sIgE test to dust mite had a sensitivity of 0.59 and a specificity of 0.93 respectively. There was a concordant result of 94% between the two tests. Conclusions Phadiatop test is a useful screening test for the diagnosis of asthma. sIgE test is further needed for the Phadiatop test positive cases.

关 键 词：支气管哮喘 CAP过敏原检测系统 PHADIATOP 特异性IGE 儿童 

分 类 号：R725.6[医药卫生—儿科]