竹叶青蛇消化道内分泌细胞的免疫组织化学定位  

IMMUNOHISTOCHEMICAL LOCALIZATION OF THE ENDOCRINE CELLS IN THE DIGESTIVE TRACT OF TRIMERESURUS STEJNEGERI

作　　者：张志强[1] 张盛周[1] 吴孝兵[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《动物学报》2001年第6期666-671,T001,共7页ACTA ZOOLOGICA SINICA

基　　金：安徽省自然科学基金资助项目 (No .990 42 419);安徽跨世纪学科带头人专项基金资助项目;安徽师范大学青年科研基金资助项目~~

摘　　要：应用 8种特异性胃肠激素抗血清对竹叶青蛇 (Trimeresurusstejnegeri)消化道内分泌细胞进行了免疫组织化学定位。 5 羟色胺细胞和生长抑素细胞在整个消化道中均有分布。 5 羟色胺细胞以十二指肠和回肠密度为最高 ,空肠、直肠和幽门其次 ,胃体较低 ,食道和贲门中偶见 ;生长抑素细胞在十二指肠处数量最多 ,胃体、幽门和空肠其次 ,其余消化道各段偶见 ;胰高血糖素细胞只见于十二指肠和空肠 ;血管活性肠肽在贲门和胃体处有少量阳性细胞分布 ,食道中偶见。以上各处内分泌细胞的分布密度差异显著。未检出胃泌素、P 物质、胰多肽和胰岛素免疫反应阳性细胞。上述内分泌细胞的分布特点可能与竹叶青的摄食习性和生活环境有关。At present, the gastrointestinal tract of reptiles has received a little attention from comparative endocrinologist and the available information being mainly limited to the crocodiles and lizards. To date there have been few studies of regulatory peptides in the gastrointestinal tract of snakes. However, Masini reported immunoreactivity for gastrin\\cholecystokinin (combined) and somatostatin in small intestinal endocrine cells of three species of snake, the Asp viper ( Vipera aspis ), the Grass snake ( Natrix natrix ) and the Viperine snake ( N. maura ), but found that immunoreactivity to pancreatic glucagon, secretin or motilin was absent. In addition, somatostatin containing cells had been detected in the stomach and small intestine of the European adder ( Viper aberus ). There is, however, no systematic study of regulatory peptides of the entire length of the digestive tract in any snake. In view of the evolutionary position of reptiles, an investigation of the gastrointestinal regulatory peptide system was considered to be of particular interest. Trimeresurus stejnegeri , a member of the reptilia suborder serpents, was studied as an example of poisonous snakes. The purpose of this study was to conduct a detailed examination of the distribution of the endocrine cells in the digestive tract of this snake. The tunica mucosa of the entire length of the digestive tract was examined to determine the types, relative frequencies and distributions of endocrine cells found there by using an immunohistochemical method (Avidin biotin peroxidase complex method). The eight gastrointestinal peptides involved, which had been reported to occur in mammals, were 5 Hydroxytryptamine, somatostatin, glucagon, vasoactive intestinal polypeptide, gastrin, substance P, pancreatic polypeptide and insulin. The results showed that four of them were observed in the digestive tract of Trimeresurus stejnegeri , namely 5 Hydroxytryptamine, somatostatin, glucagon and vasoactive intestinal polypeptide. 5 Hydroxytrypta

关 键 词：竹叶青蛇 消化道 内分泌细胞 免疫组织化学定位 

分 类 号：Q48[生物学—生理学]