中华鳖7种组织SOX基因表达的RT-PCR分析  

RT-PCR ANALYSIS ON SOX GENE OF SEVEN TISSUES FROM THE CHINESE SOFT-SHELLED TURTLE(PELODISCUS SINENSIS)

作　　者：聂刘旺[1] 单祥年[2] 汪鸣[1] 郭超文[2] 鲁晓萱[2] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]南京师范大学生命科学学院,南京210097

出　　处：《动物学报》2001年第6期718-720,共3页ACTA ZOOLOGICA SINICA

基　　金：安徽省和江苏省自然科学基金资助课题 (No 97412 0 0 2 ;No 975 0 6112 )~~

摘　　要：In this paper, RT PCR analysis on SOX gene of seven tissues from the Chinese soft shelled turtle (Pelodiscus sinensis) was studied, and SOX gene fragments of expression from the testicle, brain, heart, kidney and spleen were cloned using RT PCR products.The results show that SOX genes has specific expression in the testicle, brain, spleen, cardiac muscle and kidney and isn’t expression in muscle, liver and ovary of femel.The results of sequence reveal that the SOX genes of expression in the testicle are TSSOX1、TSSOX4 TSSOX5 and TSSOX9, and those are TSSOX2 and TSSOX4 in the brain, and this is TSSOX4 in the spleen and heart tissue, and those are TSSOX2 and TSSOX3 in the kidney tissue.This suggests that the SOX gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiation of male germ cell.In this paper, RT PCR analysis on SOX gene of seven tissues from the Chinese soft shelled turtle (Pelodiscus sinensis) was studied, and SOX gene fragments of expression from the testicle, brain, heart, kidney and spleen were cloned using RT PCR products.The results show that SOX genes has specific expression in the testicle, brain, spleen, cardiac muscle and kidney and isn't expression in muscle, liver and ovary of femel.The results of sequence reveal that the SOX genes of expression in the testicle are TSSOX1、TSSOX4 TSSOX5 and TSSOX9, and those are TSSOX2 and TSSOX4 in the brain, and this is TSSOX4 in the spleen and heart tissue, and those are TSSOX2 and TSSOX3 in the kidney tissue.This suggests that the SOX gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiation of male germ cell.

关 键 词：中华鳖 RT-PCR 序列分析 基因表达 组织SOX 胚胎发育 

分 类 号：Q344.13[生物学—遗传学] Q503