蝎毒液肽基因内含子剪接信号分析  

ANALYSIS OF SPLICING SIGNALS OF SCORPION VENOM PEPTIDE INTRONS

作　　者：朱顺义[1] 曹志贱[1] 李文鑫[1] 

机构地区：[1]武汉大学生命科学学院生物技术系,湖北武汉430072

出　　处：《生物物理学报》2001年第4期695-702,共8页Acta Biophysica Sinica

基　　金：国家自然科学基金 (39970897)资助项目

摘　　要：从中国蝎ButhusmartensiiKarsch基因组DNA中分离到两个毒液肽基因的内含子。在此基础上 ,通过编辑和分析目前已出版的蝎毒液肽基因内含子序列 ,确定了这类基因内含子剪接信号的共有序列 ,并将其与其它物种进行了比较。The genomic DNAs encoding two new venom peptides, BmTXKS1 (an short-chain K+ channel toxin-like peptide) and BmTXKS2(a defensin-like peptide) were isolated and characterized from Buthus martensii Karsch. Sequence analysis showed that they are both similar in genomic organization, which is composed of two exons and one intron located in the signal peptide (BmTXKS1) or pro-peptide (BmTXKS2) coding region. By compiling and analyzing the scorpion venom peptide introns reported so far, the consensus sequences related to intron splice were determined and compared with those of other organisms. These results are valuable to further study the splicing mechanism of scorpion venom peptide introns and for comparing the relationship between evolution and function of introns among different species.

关 键 词：蝎毒液肽 基因组织 剪接信号 内含子 

分 类 号：R996.3[医药卫生—毒理学] Q75[医药卫生—药学]