Tween-80胶束对苝醌类光敏剂基态和激发态的保护作用  

PROTECTIVE EFFECT OF TWEEN-80 MICELLE ON GROUND AND EXCITED STATES OF PERYLENEQUINONOID PHOTOSENSITIZERS

作　　者：刘卫[1] 张红雨[2,3] 陈兴荣[4] 周志祥[5] 张志义[5] 

机构地区：[1]蒙自师范高等专科学校,云南蒙自661100 [2]山东理工大学,山东省生物信息工程技术研究中心计算生物学实验室,山东淄博255091 [3]山东师范大学生物系,云南大理671000 [4]大理医学院药学系,北京100101 [5]中国科学院生物物理研究所

出　　处：《生物物理学报》2001年第4期761-766,共6页Acta Biophysica Sinica

基　　金：国家自然科学基金重点项目(39830090)

摘　　要：用Tween-80非离子型胶束增溶竹红菌甲素、乙素(HA、HB)及金丝桃蒽酮(HYP)等醌类光敏剂 ,发现该胶束对HA的基态和激发态都有保护作用。相对于含水有机体系 ,HA在胶束中基态的 pKa值升高 ,荧光量子产率增大 ,光敏反应产生的活性中间体1O2 和·OH的产额增加 ,从而提高了其光敏活性 ,这在光敏损伤作用中具有重要的生物学意义。实验还发现 ,Tween-80胶束对HB及HYP的保护作用与HA类似,而且保护作用与醌的结构有关 。In this work, hypocrellins A and B (HA, HB), and hypericin (HYP) were dispersed in Tween-80 micelle. It was found that the micelle had protective effect on ground state and excited state of HA. Compared with HA in water-contained organic solvents, the pigment in micelle had higher pKa value and higher fluorescence quantum yield. Moreover, the quantum yield of active species produced from photosensitization, 1O2 and ·OH, increased as well. As a result, the photosensitivity of HA was enhanced. The protective effect of Tween-80 micelle on HB and HYP was similar to that on HA, and the protective effect was related to the molecular structures of perylenequinonoid photosensitizers. This provided information for screening better photosensitizers for photodynamical therapy.

关 键 词：Tween-80胶束 Bei醌类光敏剂 竹红菌素 金丝桃蒽酮 光疗药物 光敏作用 保护作用 基态 激发态 

分 类 号：Q632[生物学—生物物理学] R454.2[医药卫生—治疗学]