ARDRA对植物根瘤内共生放线菌Frankia多样性的研究  

Biodiversity of Frankia strains in nodules from Alnus and Hippophae by ARDRA.

作　　者：吴少慧[1] 张惠文[1] 熊智[1] 刘忠[1] 张忠泽[1] 张成刚[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《应用生态学报》2001年第6期883-886,共4页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金 (39870 0 38);中国科学院 (K2 951 1 31 1 0 )资助项目

摘　　要：应用原核生物 16SrDNA特异性引物rD1和fD1,通过ARDRA (amplifiedribosomalDNArestrictionanalysis)法直接扩增自中国云南、东北地区赤杨属 3种植物和沙棘属 1种植物根瘤内FrankiaDNA ,得到一长约 15 0 0bp的扩增产物 .选用两种内切酶HaeⅢ、AfaⅠ联合对扩增产物进行酶切 ,得到稳定的酶切图谱 .将所测48个感染赤杨的Frankia样本区分为 3个不同的组 ,所测 43个感染沙棘的Frankia样本区分为 3个不同的组 .显示根瘤内Frankia存在丰富的遗传多样性 .S ribosomal DNAs(rDNAs) of total 91 samples of Frankia strains in root nodules from Alnus and Hippophae were ampilified by PCR with primer rD1 and fD1.A fragment about 1500bp long was obtained.The amplicons were digested by HaeⅢ and Afa Ⅰ together and run in agarose gels.As a result,48 Alnus-infected Frankia were distinguished into three different groups and 43 Hippophae-infected Frankia into three different groups,respectively.The results showed that there is great genetic diversity among the Frankia strains directly from root nodules.

关 键 词：赤杨 沙棘根瘤 FRANKIA 多样性 ARDRA法 共生放线菌 

分 类 号：Q948.122.3[生物学—植物学]