分离纯化内切β-葡聚糖苷酶和部分N-末端序列分析  

Purification,partial N-terminal sequencing and properties of an endoglucanase from Aspergillus niger

作　　者：沈志扬[1] 郭春腾[1] 邓文汉[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002

出　　处：《生物技术》2001年第6期7-9,共3页Biotechnology

摘　　要：使用硫酸铵分级沉淀、DEAE -TOYOPEARL 6 5 0M和POROS 2 0PI弱阴离子交换色谱等分离技术 ,从黑曲霉 (Aspergillusniger)发酵酶粉中分离提纯出一种新内切 β -葡聚糖苷酶 ,在非还原条件下分子量为 36kD ,还原条件为 38kD ,该酶最适pH3 5 ,最适温度 5 5℃ ,N -末端部分氨基酸序列为XXXFKCVGSMEDGAES。A novel cellulase was identified and isolated from a commercial Aspergillus niger cellulase preparation.The crude enzyme preparation,which was prepared by precipitation of the water extract of the culture of Aspergillus niger with ammonium sulfate,was further fractionated by three steps of chromatography of weak anion exchange to obtain an electrophoretically homogeneous cellulase. The molecular weight of the purified enzyme was estimated by SDS-PAGE to be 36kD under non-reduced conditions and 38kD under reduced conditions, with the optimun pH at 3.5, and the optimum temperature at 55℃. The partial N-terminal amino acid sequences of the purified enzyme was identified as XXXFKCVGSMEDGAES.

关 键 词：黑曲霉 内切β-葡聚糖苷糖 分离纯化 N-末端序列 性质 

分 类 号：Q556.2[生物学—生物化学] Q949.327.1