补料分批法高密度培养凝结芽孢杆菌  

High-density cultivation of Bacillus coagulans using fed-batch method

作　　者：刘馨磊[1] 肖春明[1] 许延涛[1] 戚薇[1] 杜连祥[1] 

机构地区：[1]天津轻工业学院食品工程系,天津300222

出　　处：《生物技术》2001年第6期20-23,共4页Biotechnology

基　　金：天津市自然科学基金重点研究项目(0 0 380 36 11)

摘　　要：研究在摇瓶条件下通过补加含有不同浓度的葡萄糖与酵母膏的料液及不同流加方式对凝结芽孢杆菌 (Bacilluscoagulans)TQ33菌体量和芽孢生成量的影响 ,确定了补料液中的葡萄糖与酵母膏比例为6 :1(w/w) ,采用将葡萄糖浓度控制在 3 0g/L～ 6 .0g/L的流加方式进行高密度培养凝结芽孢杆菌。在 5L自动发酵罐中进行补料分批培养 ,最终菌体浓度达到 4 5× 10 9cfu/mL ,芽孢浓度为 1 2× 10 9cfu/mL ,分别是分批培养的 5 9倍和 3 8倍。In shake flasks conditions, feg-batch culture was performed to investigate the effect of feeding solution with various concentration of glucose and yeast extract and the effect of three different fed-batch strategies on cell growth and spore formation of B.coagulans. The feeding method was determined which the glucose/yeast extract radio was 6:1(w/w) and the glucose concentration ranged from 3.0g/L to 6.0 g/L.High-cell-density fed-batch culture was operated in 5L auto-fermentor,the final cell concentration was 4.5×10 9cfu/mL,spore concentration was 1.2×10 9cfu/mL,which was as much as 5.9 times and 3.8 times of batch culture respectively.

关 键 词：凝结芽孢肝菌 高密度培养 补料分批培养 流加 

分 类 号：Q939.124[生物学—微生物学] Q93－335