EDAG-1,一种与造血调控密切相关新基因的分离和确认  

Isolation and Characterization of EDAG-1, A Novel Gene Related to Regulation in Hematopoietic System

作　　者：闾军[1] 许望翔[2] 汪思应[3] 詹轶群[2] 江岩[2] 蔡卫民[1] 杨晓明[2] 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所,杭州710006 [2]军事医学科学院放射医学研究所,北京100850 [3]安徽医科大学病理生理学教研室,合肥230000

出　　处：《生物化学与生物物理学报》2001年第6期641-646,共6页

基　　金：国家自然科学基金 (No .396 70 36 6 );国家杰出青年基金 (No .30 0 2 5 0 18)资助~~

摘　　要：以代表性差异显示分析技术 (RDA)获得一种在人胎肝组织中高丰度表达的胚胎发育相关基因 1(embry onicdevelopassociatedgene 1,EDAG 1) ,经筛选cDNA文库及 5′末端cDNA快速扩增技术 (rapidamplificationofcDNA 5′end ,5′RACE)获得其全长为 2 16 6bp的cDNA。该基因含编码 484个氨基酸组成蛋白质的ORF ,Blast检索表明编码蛋白质不含任何已知蛋白质功能结构域 ,与小鼠Hemogen及大鼠RP5 9基因同源。其mRNA 3′端非翻译区序列含 2拷贝AUUUA结构 ,表明该基因mRNA稳定性差。体外翻译证明该基因编码蛋白质分子量为 5 5 .3kD ,与理论预测值一致。Northern杂交及PCR检测表明该基因主要表达在成人骨髓及胚胎肝组织 ,在K5 6 2和MO7e两株人髓性白血病细胞系中也有较高丰度表达。氯高铁血红素及红细胞生成素EPO (erythropoietin)诱导K5 6 2细胞向红系分化过程中 ,EDAG 1的表达随细胞的分化迅速下调 ;这些结果提示EDAG 1可能是一种与造血细胞分化调控密切相关的新基因。A novel gene, named embryonic develop associated gene 1 ( EDAG 1) and abundantly expressed in human fetal liver tissues, was isolated by screening a human fetal liver cDNA library and the 5′RACE. The full length of EDAG 1 mRNA is 2 166 bp, with an open reading frame of 1 452 bp neucleotides, encoding a 484 amino acid protein. No domain or motif was found similar with other genes by Blast program. Two copies of AUUUA motif in 3′non translated region show instability of its mRNA. The molecular weight of the protein is 55.3 kD identified by the translation in vitro. EDAG 1 is specifically expressed in hematopoietic tissues, and is quickly down regulated during the differentiation of K562 cells induced by hemin and EPO. These results show that EDAG 1 is related to the regulation in hematopoietic system and the development of leukemia.

关 键 词：EDAG-1 K562细胞 分化 造血调控 胚胎发育相关基因 分离 

分 类 号：Q781[生物学—分子生物学]