玉米组培材料的玻璃化法超低温保存及冻后植株再生  

Cryopreservation of Maize Immature Embryos, Embryogenic Callus and Cell Sus- pension Cultures by Vitrification

作　　者：周小梅[1] 王国英[1] 敖光明[1] 赵云云[1] 

机构地区：[1]中国农业大学生物技术国家重点实验室,北京100094

出　　处：《农业生物技术学报》2001年第4期355-358,T001-T002,共6页Journal of Agricultural Biotechnology

基　　金：国家"转基因植物专项基金"资助

摘　　要：本研究针对玉米遗传转化中常用的受体材料幼胚、胚性愈伤组织和悬浮细胞系进行了玻璃化法超低温保存。对玻璃化超低温保存中的几个主要因素（预培养、过渡液处理时间、脱水时间、脱水时的温度等）进行了比较实验。结果表明：预培养可以大幅度提高悬浮细胞系超低温保存后的细胞存活率；在较短的脱水时间内致密型愈伤组织比松散型愈伤组织具有较高的存活率，随着脱水时间的延长，ＴＴＣ相对存活率一直呈上升趋势；经过玻璃化法冻存后的玉米幼胚愈伤诱导率可达７３％。冷冻保存后的幼胚和愈伤组织在恢复生长后，再经分化得到了大量的再生植株。This paper presents vitrifacation of maize immature embryos, embryogenic callus and cell suspension cultures which are usually used as materials for cereal transformation. Factors affecting cryopreservation by vitrification were studied including pre- culture, loading treatment, dehydration with PVS2 etc.. The results showed that preculture greatly increased the TTC relative survival rate of cell suspension; Type I callus have higher survival rate than Type Ⅱ callus' in short exposure time to PVS2. TTC relative survival rate of cell suspension rise continuously with the lapse of exposure time. Survival rate of 73% (by callus formation) was obtained from vitrified immature embryos. Plant regeneration was achieved from recovered two types of calli and immature embryos.

关 键 词：玉米 幼胚 愈伤组织 悬浮细胞系 玻璃化法 超低温保存 冻后植株再生 组培材料 种质 

分 类 号：S513[农业科学—作物学] S325.12