小麦不同生理状态的幼穗和幼胚盾片与诱导分化能力关系的研究  

The Study of Relation between Differentiation Ability and Different Physiological States of Infloresence and Scutellum from Bread Wheat (Triticum aestivum L.)

作　　者：何勇刚[1] 林刚[1] 刘曼西[1] 何光源[1] 

机构地区：[1]华中科技大学生命科学与技术学院中英HUST-IACR作物基因工程和基因组学联合实验室,武汉430074

出　　处：《武汉植物学研究》2001年第5期363-368,共6页Journal of Wuhan Botanical Research

基　　金：国家"九五"攻关项目资助 (编号 :96 -0 0 2 -0 2 -2 4 )

摘　　要：用植物组织培养的方法 ,研究了冬小麦品种鄂恩 1号和品系鄂 5 5 0 72不同生理状态的幼穗和幼胚盾片与诱导分化的关系。结果表明 ,长度在 0 .4～ 2 .0 cm间的幼穗和直径在0 .4～ 1 .5 mm间的盾片随生理状态不同 ,其诱导和分化频率有明显的差异 ,幼嫩的材料再生频率较高。经 SAS统计分析 ,发现 0 .5～ 1 .0 cm长的幼穗和直径为 0 .4～ 1 .1 mm的盾片是处于诱导分化的最佳生理时期的实验材料 ,平均每个胚性愈伤组织的植株再生分别达到 3 .1 7和 5 .6 3株。通过比较幼穗和幼胚盾片的植物组织培养结果 ,发现幼胚盾片比幼穗愈伤组织出现早 ,生长快 ,植株再生绿苗率高 ,这表明小麦幼胚盾片是较好的植物组织培养的材料。In order to choose the suitable target source and developmental stage of wheat explants for establishment of high frequency of plant regeneration system,inflorescence and scutellum explants were cultured in vitro conditions to study the effects of the explants physiological state on the frequencies of callus induction and plant regeneration.Three size classes of inflorescence(1.3~2.0 cm,0.8~1.2 cm,0.4~0.7 cm in length) and immature embryo scutellum(1.2~1.5 mm,0.8~1.1 mm,0.4~0.7 mm in diameter) were used in this study from two winter wheat genotypes En1 and E55072.According to statistical analysis,the results indicated that the explants showed significantly differences in terms of callus induction and plant regeneration in in vitro culture from explants with different size classes.For inflorescence,explants with 0.5~ 1.0 cm in length give better response,from which per embryogenic callus produced 3.17 plantlets.For scutellum,explants with 0.4~1.1 mm in diameter gave better response,from which per embryogenic callus generated 5.63 plantlets.

关 键 词：小麦 幼穗 幼胚盾片 生理状态 组织培养 诱导分化能力 

分 类 号：S512.1[农业科学—作物学]