从我国分离的3株蛙虹彩病毒与蛙病毒3型主要衣壳蛋白基因同源性的比较  

Comparison of Sequence Homology of Major Capsid Protein Gene of Ranavirus Frog Virus3 and Rana Grylio Virus isolates from China

作　　者：张奇亚[1] 肖枫[1] 李正秋[1] 桂建芳[1] 

机构地区：[1]中国科学院水生生物研究所,"淡水生态与生物技术"国家重点实验室,武汉430072

出　　处：《病毒学报》2001年第4期372-374,共3页Chinese Journal of Virology

基　　金：中国科学院王宽诚基金 ( 1999) ;中国科学院生物特支费 (STZ -0 0 -13);国家重点基础 ( 973)项目 (G19990 5 390 8)基金的部分资助

摘　　要：Since 1995,three viral isolates named RGV9506,RGV9807 and RGV9808 associated with frog mortality were isolated from farm-raised frogs (Rana grylio virus,RGV) in China.Both ultrastructural morphology and serological characterisitics had shown that the RGV isolates belong to genus Ranavirus. The DNA sequence analysis of the 5′end of the major capsid protein(MCP) gene of RGV isolates by PCR amplification produced a 531bp fragment.DNA templates were prepared from cells infected with different RGV isolates and the specific primers designed were based on highly conserved region at the 5′ of the gene encoding Frog virus 3(FV3) MCP, which is the typical species of the genus Ranavirus.The PCR products indicate that the nucleotide sequence of MCP gene of the RGV9506,RGV9807 and RGV9808 showed 99.6%, 99.8% and 98.4% homology respectively with the corresponding region of the MCP gene of FV3.Since 1995,three viral isolates named RGV9506,RGV9807 and RGV9808 associated with frog mortality were isolated from farm-raised frogs (Rana grylio virus,RGV) in China.Both ultrastructural morphology and serological characterisitics had shown that the RGV isolates belong to genus Ranavirus. The DNA sequence analysis of the 5′end of the major capsid protein(MCP) gene of RGV isolates by PCR amplification produced a 531bp fragment.DNA templates were prepared from cells infected with different RGV isolates and the specific primers designed were based on highly conserved region at the 5′ of the gene encoding Frog virus 3(FV3) MCP, which is the typical species of the genus Ranavirus.The PCR products indicate that the nucleotide sequence of MCP gene of the RGV9506,RGV9807 and RGV9808 showed 99.6%, 99.8% and 98.4% homology respectively with the corresponding region of the MCP gene of FV3.

关 键 词：蛙虹彩病毒 蛙病毒3型 FV3 聚合酶链式反应 衣壳蛋白基因 基因同源性 水产动物 

分 类 号：S947.2[农业科学—水产养殖]