玉米大斑病菌HT-毒素与玉米细胞的膜脂过氧化研究  

HT-TOXIN PRODUCED BY EXSEROHILUM TURCICUM AND SUPEROXIDATION OF MEMBRANE LIPID IN CORN CELL

作　　者：董金皋[1] 韩建民[1] 张利辉[1] 

机构地区：[1]河北农业大学植物病理学系,保定071001

出　　处：《微生物学通报》2001年第5期1-5,共5页Microbiology China

基　　金：河北省自然科学基金重点项目 (No 3 971 3 1;No 3 980 98);河北农业大学科技重点资助项目 (981 6计划 )

摘　　要：以土培玉米幼苗为材料 ,通过测定玉米大斑病菌HT 毒素处理后玉米叶片细胞膜透性和细胞内丙二醛 (MDA)含量变化及其与细胞内过氧化物歧化酶 (SOD)、过氧化物酶(POD)活性之间的关系来研究玉米细胞膜脂过氧化的程度。结果表明 ,HT 毒素胁迫后 ,亲和组合MDA含量上升 ,POD活性受到刺激 ,SOD活性受抑制较强 ;非亲和组合POD活性受到抑制 ,SOD活性受抑制较弱。HT 毒素对玉米叶片细胞膜有强烈的破坏作用 ,而且这种作用与毒素的浓度和毒素处理的时间呈正相关。试验结果初步推测在抗、感玉米的细胞膜上可能都有HT 毒素的结合位点 ,但毒素作用后由于它们的活性氧代谢程度不同而导致了对HTSuperoxidation of membrane lipid in corn leaf cells has been studied by researching the relationship between membrane permeability,the content of MDA,and the activity of POD and SOD under HT\|toxin stress.The experimental results showed that the content of MDA ascended,the POD activity was stimulated and SOD activity was inhibited in compatible combination,but POD activity was inhibited in incompatible combination.HT\|toxin strongly destroyed the permeability of cell membrane of corn,and there were positive correlation between toxin effect and toxin concentration and time exposure to toxin.It was conclusion that there may be toxin\|binding site in both resistant and susceptible cell membrane,and the difference in sensitivity of resistant and susceptible cells to toxin may result from their active oxygen metabolism.

关 键 词：玉米大斑病菌 HT-毒素 膜脂过氧化 MDA SOD POD 

分 类 号：S432.4[农业科学—植物病理学]