棉花枯萎病菌异核体的三个不同核型分离子DNA碱基组成及18S rDNA序列  

GC CONTENT AND 18S rDNA SEQUENCE IN THREE NUCLEAR TYPE SEGREGANTS ISOLATED FROM A HETEROKARYON OFFUSARIUM OXYSPORUM F.SP.VASINFECTUM

作　　者：李颖[1] 关国华[1] 蒋永申[1] 李秀玉[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《微生物学通报》2001年第5期6-9,共4页Microbiology China

基　　金：国家自然科学基金资助项目 (No. 3 9770 3 95 )~~

摘　　要：从棉花枯萎病菌 (Fusariumoxysporumf.sp .vasinfectum)异核体菌株Ag1 49的菌落角变处分离得到 3个培养特征和致病性差异明显的单孢分离子。分别测定了这 3个分离子的总DNA和核DNA的碱基组成 ,以及它们的 1 8SrDNA的部分序列 ( 1 477个碱基对 )。它们的总DNA (G +C)mol%差异在 0 1 %～ 0 4%之间 ;核DNA (G +C)mol%的差异在0 4%～ 0 8%范围内 ;而 1Three nuclear type segeragants were isolated from a heterokaryon of Fusarium oxysporum f.sp.vasinfectum strain Ag149.Their cultural character and pathogenicity were different.The differences of their GC content for total DNA and nuclear DNA were between 0 1% to 0 4% and 0 4% to 0 8% respectively,as measured by thermal denaturation method.The 18S rDNA partial sequences of 1477 base pairs in these segregants were identical.

关 键 词：棉花枯萎病菌 DNA碱基组成 18S RDNA序列 核型分离子 异核体 

分 类 号：S432.44[农业科学—植物病理学]