同批次细胞基质收获甲肝和麻疹病毒制备联合疫苗的方法  

PREARATION OF UNITED VACCINE OF HEPATITIS A AND MEASLES VIRUSES BY HARVESTING THE SAME BATCH OF CELL SUBSTRATE

作　　者：刘景晔[1] 刘令九[1] 屈翠波[1] 王拥军[1] 孙姝[1] 吴晓娟[1] 粱晓莉[1] 

机构地区：[1]长春生物制品研究所,吉林长春130062

出　　处：《微生物学杂志》2001年第4期19-21,共3页Journal of Microbiology

摘　　要：采用同一批次细胞基质收获甲肝和麻疹病毒。应用甲肝L A 1株和麻疹D3T11株 ,间隔 3周先后感染同一批次人胚肺二倍体细胞 2BS株 ,待两种病毒同时达到增殖高峰期时收获病毒液 (以下简称HAM )。对HAM分别进行病毒滴定、特异性检查、猴体安全性和免疫效果试验。结果显示 ,HAM的甲肝和麻疹病毒滴度与同批单价甲肝和麻疹疫苗病毒滴度 ,差异均无显著意义。该方法用于制备甲肝 麻疹联合疫苗 ,省时省力 ,操作简便 。Hepatitis A and measles viruses in the same batch of cell substrate were harvested applying hepatitis A virus L A 1 strain and measles virus D3T11 strain. An interval of 3 weeks the two viruses were infected successively to human lung diploid fibroblast cell of the same batch of 2BS strain. When the two viruses have reached to their proliferating peak simultaneously, they were harvested in liquid state (HAM for short). The HAM was detected on virus titer, specificity, safety in monkey, and immune effect respectively. The results showed that the united vaccine of hepatitis A and measles in HAM has no significant difference as compared with single vaccines in the same batch substrate. Therefore, this preparation method of united vaccine is superior to routine one because of time saving, work saving, easy operating, and cost reducing.

关 键 词：甲肝 麻疹 细胞基质 联合疫苗 制备方法 

分 类 号：R392.7[医药卫生—免疫学] R392－33[医药卫生—基础医学]