RT套式PCR检测血浆HCV RNA及与抗HCV检测的比较  

DETECTION OF HCV RNA IN PLASMA BY DIRECT RT-NEST PCR AND ITS COMPARISON WITH HCV ANTIBODY DETECTION

作　　者：赵继义[1] 刘雪梅 郭薇媛[3] 高磊[1] 钟照华[1] 谷鸿喜[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,黑龙江哈尔滨150086 [2]哈尔滨市道里区防疫站,黑龙江哈尔滨150010 [3]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086

出　　处：《微生物学杂志》2001年第4期37-38,共2页Journal of Microbiology

摘　　要：应用微量血清热变性法提取核酸 ,逆转录套式聚合酶链反应 (RT nestPCR)检测血浆HCVRNA ,并与抗HCVELISA检测结果比较 ,对HCVRNA阳性标本进行HGVRNA的筛查。结果在 32例抗HCV阳性和 2 0例抗HCV阴性血浆中 ,HCVRNA分别检出 18例和 2例 ,总符合率为 70 % ,2 0例HCVRNA阳性者中有 2例合并感染HBV ,1例合并感染HGV。HCV RNA in plasma had been detected by a mini heat denatured RNA extraction and RT nest PCR. HBV DNA and HGV RNA has also been tested in the HCV RNA positive samples. Among 32 cases of HCV antibody positive and 20 of HCV antibody negative, 18 samples (56.5%) and 2 samples (10%) were HCV RNA positive respectively. Two HCV RNA positive samples were found HBV DNA positive, whereas 1 HCV RNA positive sample was showed HGV RNA positive. It is proved there is a good interrelation between HCV and anti HCV, not parallel though.

关 键 词：丙型肝炎病毒 逆转录套式PCR 血浆 HCVRNA 检测 抗体 

分 类 号：R512.63[医药卫生—内科学] R446.62[医药卫生—临床医学]