HPLC法测定白芥子药材中芥子碱硫氰酸盐的含量  

Determaination of Sinapine Thiocyanate in Semen Sinapis alba (Bai Jiezi) by HPLC

作　　者：刘丽芳[1] 王宇新[1] 刘雪芳[1] 张新勇[2] 

机构地区：[1]中国药科大学,南京210038 [2]江苏省药物研究所,南京210009

出　　处：《中国野生植物资源》2001年第5期49-50,28,共3页Chinese Wild Plant Resources

摘　　要：本文采用RP—HPLC法测定了不同产地白芥子药材中芥子碱硫氰酸盐的含量 ,方法为 :色谱柱 :Allti maPhenyl,5 μ ,2 5 0× 4.6mm ;流动相 :乙腈— 0 0 8mol/L磷酸二氢钾溶液 (15 :85 ) ,检测波长 :32 6nm ;流速 :1mL/min。柱温 :室温。实验表明 ,该法快速、简便 ,具有很好的重现性和稳定性。We determained the sinapine thiocyanate in Seman Sinapis Albae (Bai Jiezi) from different places by RP-HPLC. The column employed was Alltima Phenyl Column (250×4.6mm,5um).The mobile phase was acetonitrile-0.08Mol/LKH 2PO 4(15∶85). The flow rate was 1.0mL/min and detection was effected at 326nm. The results show the method is accurate, rapid and reproducible.

关 键 词：白芥子 芥子碱硫氰酸盐 HPLC 中药材 含量测定 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]