HLA-Ⅰ、Ⅱ类PCR-SSP基因分型在肾移植配型及亲子鉴定中的应用  

Application of PCR-SSP In Kidney Transplantation Typing and Consanguinity Identification

作　　者：周永安[1] 杨波[1] 马瑞林[1] 朱镭[1] 张丽[1] 鹿育晋[1] 

机构地区：[1]山西医科大学第二医院,太原030001

出　　处：《中国中西医结合肾病杂志》2001年第12期710-711,714,共3页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的 :建立快速、准确的HLA基因分型方法 ,满足临床移植配型的需要。方法 :对 2 1对已进行HLA抗原血清学分型的肾移植的供、受者 ,12例亲子鉴定应用聚合酶链反应 -序列特异引物 (PCR -SSP)进行HLA -Ⅰ、Ⅱ类基因A、B、DRB1、DQB1位点扩增 ,琼脂糖凝胶电泳分析PCR产物并确定其基因型。结果 :基因分型结果与血清学分型一致者有 18对 ,基因分型能明确判断而血清学分型无法判断者有 3对 ;排除亲子关系的有两例。结论 :PCR -SSP方法具有操作简单、快速、结果可靠的特点 ,不仅适用于移植配型、法医学亲子鉴定和个人识别 。Objective: To establish a rapid, accurate HLA DNA typing method for transplantation matching. Methods: HLA genotyping was performed by using PCR-SSP in 21 pairs of kidney transplantation donor-recipients who underwent serologic typing and in 12 consanguinity identifications. Genotype was identified by analysis of amplification product agarose gel electrophoresis. Results: The serological and genotyping results were identified in 18 cases. Serologic typing was failed in 3 cases who could be typed easily by genotyping methods. Two cases of non-consanguinity were identified. Conclusion: This typing method is simple, fast, accurate and reliable. It can used for transplantation typing and consanguinity identification in medical jurisprudence and individual identifying, as well as for studying related diseases and human heredity genetics.

关 键 词：HLA PCR-SSP 移植配型 亲子鉴定 

分 类 号：R699.2[医药卫生—泌尿科学] D919.4[医药卫生—外科学]