兔角膜内皮细胞体外原代培养及形态学观察  

Experimental study on the prime-cultured corneal endothelial cells and observation in rabbits

作　　者：袁进[1] 周文天[1] 周辉[1] 兰绪达[1] 

机构地区：[1]中国江西医学院第二附属医院眼科,330006

出　　处：《美中国际眼科杂志》2001年第3期17-19,共3页

摘　　要：目的 研究培养兔角膜内皮细胞,为实验提供基础。方法 通过改良的细胞收获、培养技术,在体外进行兔角膜内细胞的培养,细胞的类型由电镜证实。观察hEGF不同时间点对角膜内皮细胞生长状态的影响。结果 未加入任何促细胞裂剂的情况下,细胞在体外生长良好,约一周左右形成密集状态,形态类似天然细胞,经倒置显微镜和电镜证实为角膜内细胞。在有hEGF存在时,细胞生长迅速,3-4天形成单层,各个时间点EGF组的细胞数高于对照组。结论 该技术可为角内皮细胞的研究提供较多的纯内皮细胞。Objective To provide a sound basis for experimental research by cultivation of endothelial cells from rabbit cornea. Methods A modified technique of harvesting and culturing CE from rabbit donor corneas were used, and the cells cultivated in vitro, the type of the cells were identified by electron microscope. At the same time the cultured CE was observed and cell counts were determined between experimental group containing medium hEGF 100 ng/ml and controlled group without hEGF. Results The cells grew well without adding any mitogen in the tissue culture medium and attained confluency about 1 week, their morphology resembled natural CE. The cultured cells were confirmed as corneal endothelial cells under inverted microscope and electron microscope. The cells of experimental group containing medium hEGF 100 ng/ml grew faster than control group . Conclusion With this technique, sufficient quantities of normal live cells become available for research of CE.

关 键 词：内皮细胞 角膜 细胞培养 电镜 细胞形态学 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]