桔梗悬浮培养对细胞天麻素的生物转化  

BIOTRANSFORMATION OF GASTRODIN BY CELL SUSPENSION CULTURE OF PLATYCODON GRANDIFLORUM

作　　者：戴均贵[1] 鲁丹丹[1] 崔亚君[1] 郭洪祝[1] 郑俊华[1] 果德安[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100083

出　　处：《药学学报》2001年第12期942-943,共2页Acta Pharmaceutica Sinica

基　　金：国家杰出青年基金 ( 3992 5 0 40 ) ;教育部跨世纪优秀人才基金资助项目

摘　　要：AIM To modify the structure of gastrodin using cell suspension cultures of Platycodon grandiflorum as biocatalyst. METHODS Using plant tissue and cell culture techniques to establish cell suspension cultures, and chromatographic and spectral techniques to isolate and identify the product. RESULTS 1) The medium of MS + 5 0 mg·L -1 NAA was good for the induction and growth of the callus cultures, and that of MS + 0 5 mg·L -1 NAA + 0 5 mg·L -1 6 BA + 0 2 mg·L -1 2,4 D for the growth of suspended cell cultures. 2) Gastrodin was bioconverted to p hydroxy methyl phenol, the deglucosylderivative, in good yield by cell suspension cultures of Platycodon grandiflorum . CONCLUSION Biotransformation using plant cell suspension cultures is an approach to structural modification of some natural products.AIM To modify the structure of gastrodin using cell suspension cultures of Platycodon grandiflorum as biocatalyst. METHODS Using plant tissue and cell culture techniques to establish cell suspension cultures, and chromatographic and spectral techniques to isolate and identify the product. RESULTS 1) The medium of MS + 5 0 mg·L -1 NAA was good for the induction and growth of the callus cultures, and that of MS + 0 5 mg·L -1 NAA + 0 5 mg·L -1 6 BA + 0 2 mg·L -1 2,4 D for the growth of suspended cell cultures. 2) Gastrodin was bioconverted to p hydroxy methyl phenol, the deglucosylderivative, in good yield by cell suspension cultures of Platycodon grandiflorum . CONCLUSION Biotransformation using plant cell suspension cultures is an approach to structural modification of some natural products.

关 键 词：桔梗 天麻素 生物转化 悬浮培养细胞 中药 

分 类 号：R282.710.3[医药卫生—中药学]