醛固酮介导自发性高血压大鼠心肌细胞肥大  

MYOCYTE HYPERTROPHY INDUCED BY ALDOSTERONE IN SPONTANEOUS HYPERTENSIVE RATS

作　　者：谢晓华[1] 刘宁[2] 刘秀华[1] 陈鸣和[3] 唐朝枢[4] 王士雯[1] 

机构地区：[1]解放军总医院,北京100853 [2]天津解放军第272医院 [3]广州军区广州总医院 [4]北京大学医学部

出　　处：《解放军医学杂志》2001年第12期884-886,共3页Medical Journal of Chinese People's Liberation Army

基　　金："十五"军队医药卫生科研基金资助课题 (编号 0 1MA1 2 2 )

摘　　要：为探讨醛固酮在高血压心肌细胞肥大中的作用 ,测定了自发性高血压大鼠 (spontaneouslyhyperten siverats ,SHRs)、螺内酯治疗SHRs和正常血压WKY大白鼠中左心室重与心重比值、醛固酮浓度、丝裂素活化蛋白激酶活性 (mitogen activatedproteinkinases ,MAPKs)及 4、6、8h 3个培养时间点心肌3 H 亮氨酸 ( 3 H leucine ,3 H leu)掺入量等。结果显示 ,SHRs左心室重与心重比值、各时间点心肌3 H leu掺入量及MAPKs活性均明显升高 ,螺内酯治疗可明显降低SHRs左心室重与心重比值、各时间点心肌3 H leu掺入量、血浆醛固酮浓度及MAPKs活性。提示醛固酮与其心脏受体结合并激活MAPKs ,进而介导SHRs心肌细胞蛋白合成增加及心肌细胞肥大。To detect the effect of the aldosterone(ald) on the development of myocyte hypertrophy in SHRs, the left ventricle-heart weight ratio (LVR), the plasma concentrations and the cardiac contents of ald, the activity of cardiac MAPKs were measured in WKY rats and in SHRs either untreated and treated with spironolactone (spiron), respectively. The incorporations of 3 H-leu in incubated myocardial tissue slices were also detected in the above groups at three incubated time points of 4, 6, 8h. The results showed that the LVR, the cardiac corporations of 3 H-leu at three time points,and the activity of MAPKs were significant increases in SHRs, whereas the LVR, the corporations of 3 H-leu at each time point, the activity of MAPKs, and the levels of plasma ald were significant deceased in spiron treated SHRs. The data suggested that the combination of the local cardiac tissue ald with its receptor could induce increases in the synthesis of the myocyte proteins,leading to myocyte hypertrophy, in SHRs by activating the cardiac MAPKs.

关 键 词：高血压 醛固酮 螺内酯 心肌肥大 动物实验 

分 类 号：R544.1[医药卫生—心血管疾病]