氧化低密度脂蛋白功能性上调人脐带静脉内皮细胞的生长调节致癌基因α  

oxLDL FUNCTIONALLY UPREGULATES GROWTH-REGULATED ONCOGENE α EXPRESSION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

作　　者：雷著斌[1] 刘建平[1] 李晓燕[1] 

机构地区：[1]济南军区总医院,济南250031

出　　处：《解放军医学杂志》2001年第12期907-909,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军"十五"医药卫生科研基金资助课题 (编号 0 1Q0 1 5)

摘　　要：探讨趋化因子生长调节致癌基因α(GROα)在低密度脂蛋白诱导动脉粥样硬化过程中的作用。采用人脐带静脉内皮细胞和单核细胞系细胞———U 937细胞。结果显示 ,内皮细胞暴露于氧化低密度脂蛋白后显著上调GROαmRNA ;氧化低密度脂蛋白时间、浓度依赖性地上调内皮细胞表面GROα的蛋白表达 ,但溶液中的GROα蛋白并没有显著改变。内皮细胞表面GROα蛋白上调增加了单核细胞粘附到内皮细胞 ,且GROα抗体抑制此粘附过程。提示氧化低密度脂蛋白功能性上调人脐带静脉内皮细胞GROα的表达。To study the effect of chemokine growth-regulated oncogene α (GROα) in process of LDL-induced atherosclerosis, a monocytic cell line, U937 cells and human umbilical vein endothelial cells were used. GROα mRNA levels were significantly elevated after exposure of endothelial cells to oxLDL. A significant upregulation of GROα surface expression in endothelial cells was induced by oxLDL with concentration-dependent and time-dependent manners, but no significant change in GROα was observed in the medium. Upregulation of GROα expression in endothelial cells enhanced monocytic cells adhesion to endothelial cells, but antibodies to GROα inhibited the adhesion process. oxLDL functionally upregulates GROα expression in endothelial cells.

关 键 词：氧化低密度脂蛋白 生长调节致癌基因a 内皮细胞 单核细胞 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]