烫伤大鼠组织白介素-1β转换酶mRNA的表达及其对IL-18和Fas配体的影响  

The dynamic change of ICE mRNA expression and its effect on IL-18 and FasL in thermal injured rats

作　　者：刘京衢[1] 高维谊[1] 姚咏明[1] 柴家科[1] 郭振荣[1] 盛志勇[1] 

机构地区：[1]解放军304医院烧伤研究所,北京100037

出　　处：《军医进修学院学报》2001年第4期277-280,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家重点基础研究发展规划资助项目 (G19990 5 42 0 3)

摘　　要：目的 :探讨烫伤后肠、肺组织白介素 1β转换酶 (IL 1βconvertingenzyme ,ICE)mRNA的表达对IL 18、Fas配体mRNA表达的影响。方法 :采用大鼠 30 %体表面积Ⅲ度烫伤模型 ,组织ICE、IL 18、FasLmRNA含量采用逆转录聚合酶链式反应检测。结果 :烫伤后组织ICE、IL 18、FasLmRNA表达较伤前值即有显著升高 ,烫伤后 8h达高峰且一直持续至伤后 2 4h ,给予SDD预治疗后可不同程度抑制ICE、IL 18、FasLmRNA的表达。相关分析显示 ,组织ICE、IL 18、FasLmRNA表达呈显著正相关关系。结论 :组织ICEmRNA表达在烫伤早期即显著增多 ,并呈一逐渐升高的趋势 ,对IL 18。Objective:To investigate the dynamic changes of ICE mRNA expression and the relationship between ICE mRNA and IL 18?FasL in thermal injured rats after delayed resuscitation. Methods:The animals were subjected to a 30%total body surface area full thickness thermal injury,fluid resuscitation were performed at 6 hours post burns 54 male Wistar were randomly divided into three groups: normal controls,30% Ⅲ injury group,and selective decontamination of the digestive tract (SDD)treatment group Tissue ICE? IL 18? FasL mRNA expression was determined by reverse transcription po1ymerase chain reaction (RT PCR). Results: ICE?IL 18?FasL mRNA expression significantly increased at 2 hours,peaking at 8 hours,and a high level was maintained till 24 hours. However, treatment with SDD could markedly inhibit ICE?IL 18?FasL mRNA expression in various organs.In addition, positive correlation between ICE mRNA and IL 18?FasL mRNA expression were noted( r =0 736～0 864, P <0 01).Conclusion: This study suggests that major burns can lead to a marked elevation of tissue ICE mRNA expression in vital organs,which might be associated with IL 18?FasL mRNA expression.

关 键 词：细胞介素1β转换酶 白细胞介素18 FAS配体 烫伤 动物实验 

分 类 号：R644[医药卫生—外科学]