哈密瓜细菌性果斑病病原菌鉴定  

PATHOGEN IDENTIFICATION OF HAMI MELON BACTERIAL FRUIT BLOTCH

作　　者：赵廷昌[1] 孙福在[1] 王兵万[1] 回文广[1] 

机构地区：[1]中国农业科学院植物保护研究所

出　　处：《植物病理学报》2001年第4期357-364,共8页Acta Phytopathologica Sinica

摘　　要：20 0 0年在内蒙古和新疆的哈密瓜上发现一种新细菌病害—哈密瓜细菌性果斑病 ,从病叶和病果上分离到 33个细菌菌株 ,接种哈密瓜、西瓜和甜瓜后 ,发病症状与自然发病症状完全一致 ,而且从接种病株上又重新分离到了此病原细菌 ,这 33个细菌菌株经柯赫法则证明均为该病的致病菌。各菌株致病力无明显差异。经革兰氏染色反应、菌体形态、培养性状、生理生化反应、细胞化学成分分析(糖、蛋白质、氨基酸、脂肪酸、mol% G+ C)、DNA- DNA杂交 ,确认该病原菌为燕麦食酸菌西瓜亚种(Acidovorax avenae subsp. citrulli Willems et al. 1992 ) =类产碱假单胞菌西瓜亚种 (Pseudomonaspseudoalcaligenes subsp.citrulli Schaad et al.1978)。该病菌除侵染哈密瓜外 ,人工接种尚能侵染多种葫芦科及番茄。A new bacterial disease of Hami melon named as bacterial fruit blotch was first found in Neimenggu and Xinjiang provinces of China in 2000. Thirty three strains were isolated from diseased fruits and leaves of Hami melon. Inoculation on young Hami melon and waterme lon seedlings and watermelon and muskmelon fruits with these isolates produced the same symptom as naturally infected plants. All of 33 isolates were identified as Acidovorax avenae subsp. citrulli Willems,Goor,Thieiemans,Gillis,Kersters & Ley(= Pseudomonas pseudoalcaligenes subsp. citrulli Schaad, Sowell, Goth, Colwell &Webb) by pathogenicity, stains reaction, morphological characterization, colony pattern, physiological and biochemical reactions, cytochemical analysis (saccharides, proteins, amino acids,fatty acids and mol%G+C), and DNA DNA hybridization. Inoculations with these strains on leaves of cucumber, Chinese waxgourd, pumpkin, summer squash, watermelon, muskmelon, luffa, balsam pear, vegetable sponge, calabash gourd, green calabash, tomato, and eggplant produced spot symptoms.

关 键 词：燕麦食酸菌西瓜亚种 哈密瓜果斑病 致病性 鉴定 病原菌 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]