饲用植物诸葛菜下胚轴高频率植株再生研究  

Influential factors for high freguency plant regeneration from hypocotyls of Orychophragmus violaceus,a forage resource

作　　者：陈艳红 罗鹏[1] 柏正强[2] 

机构地区：[1]四川大学植物研究所,成都610064 [2]四川省草原研究所,红原624400

出　　处：《四川草原》2001年第3期50-52,共3页Journal of Sichuan Grassland

摘　　要：诸葛菜茎叶富含蛋白质 ,是优良饲料植物。以生长于两种不同MS培养基上的诸葛菜无菌苗下胚轴为外植体 ,研究不同外植体 ,不同激素组合对下胚轴高频率再生的影响效应。实验结果如下 :激素的影响效应最显著 ,最适激素组合为MS + 6-BA 1mg/L +NAA 0 5mg/L ,植株再生频率平均高达 92 5 1 %。不同外植体作用效应的差异只表现于培养基MB1N和MB2N。Hypocotyls of different sterilized seedlings of O.violaceus were cultured to study the effects of explant and hormone on the frequency of shoot regeneration.The results were as follows.The effect of hormone was the most significant,the suitable hormone combination was 1mg/L 6-BA+0.5mg/L NAA.The highest shoot regeneration frequency was 92.5%.At the same time,the effect of explants was observed only on media MB2N and MB1N.Explants from the MS medium containing hormone showed high shoot regeneration frequency on both MB2N and MB1N,but other explants showed only high shoot regeneration frequency on the suitable medium MB1N.

关 键 词：诸葛菜 下胚轴 植株再生率 影响因素 饲用植物 

分 类 号：S548[农业科学—作物学] Q945.39[生物学—植物学]